Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education; Institute of Environmental Research at Greater Bay , Guangzhou University , Guangzhou 510006 , China.
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry/KLGHEI of Environment and Energy Chemistry, School of Chemistry , Sun Yat-sen University , Guangzhou 510275 , China.
Anal Chem. 2018 Jul 17;90(14):8607-8615. doi: 10.1021/acs.analchem.8b01855. Epub 2018 Jul 2.
Herein, the ultrathin and robust diphenylalanine (FF) self-assembled nanosheets were fabricated by a gold-stabilized strategy for the first time, using a facile electrospray method followed by a thermal treatment process. The key for the gold-stabilized mechanism was explored, demonstrating that the synergy of the stable binding and steric effect between gold nanoparticles (AuNPs) and the exposed amino groups of FF nanosheets, led to strong thermal stability and solvent resistance of the composites. Contributing to the features of remarkable accessible surfaces and strong laser light absorption ability of this FF/Au nanosheets, two robust functional devices, that is, solid-phase microextraction (SPME) fiber and surface-assisted laser desorption/ionization (SALDI) platforms, were in situ prepared for in vitro and in vivo biological analysis. The findings indicated that the fabricated platforms possessed two advantages: (1) rapid absorption/desorption speed (within 5 min) and (2) remarkable enhancement of ionization efficiency with 2 orders of magnitude. As a result, the extraction efficiency of the SPME fiber and the quantitation ability of SALDI platform were significantly improved. This study not only demonstrated that FF/Au composites could be prepared through an electrospray method followed with thermal-treatment to serve as promising adsorption/desorption/ionization materials for specific applications but also provided useful strategy to advance the ideas for future combination of SPME with LDI technique.
本文首次通过一种简便的电喷方法结合热处理过程，利用金稳定策略制备了超薄且坚固的二苯丙氨酸（FF）自组装纳米片。探讨了金稳定机制的关键，表明金纳米粒子（AuNPs）与 FF 纳米片暴露的氨基之间稳定结合和空间位阻效应的协同作用，导致了复合材料具有很强的热稳定性和耐溶剂性。由于 FF/Au 纳米片具有显著的可及表面和强激光吸收能力的特点，原位制备了两种坚固的功能器件，即固相微萃取（SPME）纤维和表面辅助激光解吸/电离（SALDI）平台，用于体外和体内生物分析。研究结果表明，所制备的平台具有两个优点：（1）快速的吸收/解吸速度（在 5 分钟内）；（2）离子化效率显著提高了 2 个数量级。因此，SPME 纤维的萃取效率和 SALDI 平台的定量能力得到了显著提高。本研究不仅证明了可以通过电喷法结合热处理制备 FF/Au 复合材料，作为特定应用的有前途的吸附/解吸/离子化材料，还为未来将 SPME 与 LDI 技术结合提供了有用的策略。
