Kanofsky J R, Wright J, Tauber A I
FEBS Lett. 1985 Aug 5;187(2):299-301. doi: 10.1016/0014-5793(85)81263-1.
We have previously studied purified human myeloperoxidase-hydrogen peroxide-halide ion systems as models of possible singlet oxygen production by granulocytes. While myeloperoxidase could efficiently produce singlet oxygen, the yield of singlet oxygen at a physiological pH with Cl- was very small due to enzyme inactivation. In that Bolscher et al. [(1984) Biochim. Biophys. Acta 784, 189-191] observed that micromolar concentrations of ascorbic acid prevented inactivation of myeloperoxidase and increased the production of hypochlorous acid, we examined whether ascorbic acid would augment singlet oxygen production by the myeloperoxidase-hydrogen peroxide-halide ion systems. Ascorbic acid, however, fails to increase the singlet oxygen yield, suggesting that it does not augment singlet oxygen production in the intact granulocyte by a myeloperoxidase-dependent mechanism.
我们之前研究了纯化的人髓过氧化物酶-过氧化氢-卤离子系统，以此作为粒细胞产生单线态氧的可能模型。虽然髓过氧化物酶能够高效产生单线态氧，但在生理pH值下，由于酶失活，以Cl-作为卤离子时单线态氧的产量非常低。鉴于博尔舍尔等人[（1984年）《生物化学与生物物理学报》784卷，第189 - 191页]观察到微摩尔浓度的抗坏血酸可防止髓过氧化物酶失活并增加次氯酸的产生，我们研究了抗坏血酸是否会增强髓过氧化物酶-过氧化氢-卤离子系统产生单线态氧的能力。然而，抗坏血酸未能提高单线态氧的产量，这表明它不会通过依赖髓过氧化物酶的机制增强完整粒细胞中单线态氧的产生。
