Kenzaka Takehiko, Yasui Madoka, Baba Takashi, Nasu Masao, Tani Katsuji
Faculty of Pharmacy, Osaka Ohtani University.
Graduate School of Pharmaceutical Sciences, Osaka University.
Biocontrol Sci. 2018;23(2):53-59. doi: 10.4265/bio.23.53.
Recent studies have shown that the genome of Legionella pneumophila is characterized by many foreign genes from a variety of eukaryotes. The eukaryotic like proteins are known to play a role in its multiplication within host cells; however, their evolutionary genetics of L. pneumophila in environments is unknown. In this study, we examined the nonsynonymous/synonymous substitution rate of eukaryotic like domain encoding genes among L. pneumophila strains. In silico analysis revealed that the nonsynonymous/synonymous substitution rate in F-box domain gene (lpp0233) was higher than those in other eukaryotic like domain and protein encoding genes and housekeeping genes. The F-box domain gene sequences in L. pneumophila strains isolated from a natural hot spring were more diversified than those of previously known strains, owing to preferential positive selection.
最近的研究表明，嗜肺军团菌的基因组具有许多来自各种真核生物的外源基因。已知类似真核生物的蛋白质在其在宿主细胞内的繁殖中起作用；然而，嗜肺军团菌在环境中的进化遗传学尚不清楚。在本研究中，我们检测了嗜肺军团菌菌株中编码类似真核生物结构域的基因的非同义/同义替换率。电子分析显示，F-box结构域基因（lpp0233）中的非同义/同义替换率高于其他类似真核生物结构域、蛋白质编码基因和管家基因。由于优先的正选择，从天然温泉分离的嗜肺军团菌菌株中的F-box结构域基因序列比先前已知的菌株更加多样化。
