利用激光多普勒成像与监测技术分析大鼠脊髓微循环
Using Laser Doppler Imaging and Monitoring to Analyze Spinal Cord Microcirculation in Rat.
作者信息
Jing Yingli, Bai Fan, Chen Hui, Dong Hao
机构信息
China Rehabilitation Research Center; Institute of Rehabilitation Science of China; Center of Neural Injury and Repair, Beijing Institute for Brain Disorders; Beijing Key Laboratory of Neural Injury and Rehabilitation.
China Rehabilitation Research Center; Institute of Rehabilitation Science of China; Center of Neural Injury and Repair, Beijing Institute for Brain Disorders; Beijing Key Laboratory of Neural Injury and Rehabilitation;
出版信息
J Vis Exp. 2018 May 30(135):56243. doi: 10.3791/56243.
Laser Doppler flowmetry (LDF) is a noninvasive method for blood flow (BF) measurement, which makes it preferable for measuring microcirculatory alterations of the spinal cord. In this article, our goal was to use both Laser Doppler imaging and monitoring to analyze the change of BF after spinal cord injury. Both the laser Doppler image scanner and the probe/monitor were being employed to obtain each readout. The data of LDPI provided a local distribution of BF, which gave an overview of perfusion around the injury site and made it accessible for comparative analysis of BF among different locations. By intensely measuring the probing area over a period of time, a combined probe was used to simultaneously measure the BF and oxygen saturation of the spinal cord, showing overall spinal cord perfusion and oxygen supply. LDF itself has a few limitations, such as relative flux, sensitivity to movement, and biological zero signal. However, the technology has been applied in clinical and experimental study due to its simple setup and rapid measurement of BF.
激光多普勒血流仪（LDF）是一种用于测量血流（BF）的非侵入性方法，这使得它在测量脊髓微循环改变方面更具优势。在本文中，我们的目标是使用激光多普勒成像和监测来分析脊髓损伤后血流的变化。激光多普勒图像扫描仪和探头/监测仪都被用于获取每个读数。激光多普勒血流灌注成像（LDPI）的数据提供了血流的局部分布，概述了损伤部位周围的灌注情况，并便于对不同位置的血流进行比较分析。通过在一段时间内密集测量探测区域，使用组合探头同时测量脊髓的血流和氧饱和度，显示脊髓的整体灌注和氧供应情况。LDF本身有一些局限性，如相对通量、对运动的敏感性和生物零信号。然而，由于其设置简单且能快速测量血流，该技术已应用于临床和实验研究。
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