Kuliga Katarzyna Z, McDonald Erin F, Gush Rodney, Michel Charles, Chipperfield Andrew J, Clough Geraldine F
Human Development & Health, Faculty of Medicine, University of Southampton, Southampton, UK.
Microcirculation. 2014 Aug;21(6):562-73. doi: 10.1111/micc.12136.
To evaluate the dynamics of skin microvascular blood flow (BF) and tissue oxygenation parameters (OXY) measured simultaneously at the same site using a combined non-invasive BF+OXY+temperature probe.
Skin BF, oxygenated (oxyHb) and deoxygenated (deoxyHb) haemoglobin and mean oxygen saturation (SO2 ) were measured in 50 healthy volunteers at rest and during perturbation of local blood flow by post-occlusive reactive hyperaemia, sympathetic nervous system-mediated vasoconstriction (deep inspiratory breath-hold) and local skin warming. Signals were analysed in time and frequency domains.
The relationship between BF and SO2 over the range of flows investigated was described by a non-linear equation with an asymptote for SO2 of 84% at BF >50 PU. SO2 was independently associated with BF, skin temperature, BMI and age, which together identified 59% of the variance in SO2 (p<0.0001). Fourier analysis revealed periodic low frequency fluctuations in both BF and SO2 , attributable to endothelial (0.01 Hz), neurogenic (0.04 Hz) and myogenic (~0.1 Hz) flow motion activity. The frequency coherence between the BF and SO2 signals was greatest in the endothelial and neurogenic frequency bands.
The simultaneous evaluation of microvascular blood flow and oxygenation kinetics in healthy skin provides a platform from which to investigate microvascular impairment in the skin and more generally the pathogenesis of microvascular disease.
使用无创性血流+组织氧合+温度联合探头，评估在同一部位同时测量的皮肤微血管血流（BF）和组织氧合参数（OXY）的动态变化。
对50名健康志愿者在静息状态下以及通过阻断后反应性充血、交感神经系统介导的血管收缩（深吸气屏气）和局部皮肤升温对局部血流进行干扰时，测量皮肤BF、氧合血红蛋白（oxyHb）、脱氧血红蛋白（deoxyHb）和平均氧饱和度（SO2）。对信号进行时域和频域分析。
在所研究的血流范围内，BF与SO2之间的关系由一个非线性方程描述，当BF>50 PU时，SO2的渐近值为84%。SO2与BF、皮肤温度、BMI和年龄独立相关，这些因素共同解释了SO2中59%的变异（p<0.0001）。傅里叶分析显示BF和SO2中均存在周期性低频波动，这归因于内皮（0.01 Hz）、神经源性（0.04 Hz）和肌源性（~0.1 Hz）的血流运动活动。BF和SO2信号之间的频率相干性在内皮和神经源性频段最大。
对健康皮肤中微血管血流和氧合动力学的同步评估提供了一个平台，可据此研究皮肤中的微血管损伤以及更广泛的微血管疾病发病机制。
