Chan P T, Ohmori H, Tomizawa J, Lebowitz J
J Biol Chem. 1985 Jul 25;260(15):8925-35.
The primary structure of the plasmid ColE1 DNA has been determined. The plasmid DNA consists of 6646 base pairs (molecular mass of 4.43 MDa) and is 48.46% in GC content. The phi 80 trp insert of the composite plasmid of ColE1, pVH51, has also been determined. The determination of the nucleotide sequence of ColE1 DNA provides the basis for examining the relationships between the DNA sequence and the gene organization of the plasmid. The focus of this paper is to use this sequence data coupled with a review of the literature and our own work to examine the nine known functional regions of ColE1: imm (colicin E1 immunity), rep (replication function), inc (plasmid incompatibility and copy number control), bom (basis of mobility), rom (modulator of inhibition of primer formation by RNA I), mob (plasmid mobilization), cer (determinant for conversion of plasmid multimers to monomers), exc (plasmid entry exclusion), cea (structural gene for colicin E1), and kil (structural gene for the Kil protein).
质粒ColE1 DNA的一级结构已被确定。该质粒DNA由6646个碱基对组成（分子量为4.43 MDa），GC含量为48.46%。ColE1的复合质粒pVH51中的φ80 trp插入片段也已被确定。ColE1 DNA核苷酸序列的测定为研究DNA序列与质粒基因组织之间的关系提供了基础。本文的重点是利用这些序列数据，结合文献综述和我们自己的研究工作，来研究ColE1的九个已知功能区域：imm（大肠杆菌素E1免疫性）、rep（复制功能）、inc（质粒不相容性和拷贝数控制）、bom（移动性基础）、rom（RNA I对引物形成抑制作用的调节因子）、mob（质粒动员）、cer（质粒多聚体转化为单体的决定因素）、exc（质粒进入排斥）、cea（大肠杆菌素E1的结构基因）和kil（Kil蛋白的结构基因）。
