Yu Xiaozheng, Li Chunyun, Song Hechao, Wang Xueyan, Guo Yinan, Cui Lijian, Liu Xiaoya, Chi Cheng, Liu Ruixia, Yin Chenghong
From the Department of Internal Medicine, Beijing Obstetrics and Gynecology Hospital.
Department of emergency, China Rehabilitation Research.
Pancreas. 2018 Aug;47(7):892-897. doi: 10.1097/MPA.0000000000001080.
The aim of this study was to investigate the effects of emodin on attenuating autophagy response in acute pancreatitis (AP) models.
Acute pancreatitis was induced in Wistar rats by injecting 3% sodium taurocholate into the biliopancreatic duct. Emodin (40 mg/kg per day) was then given intragastrically, administrated 2 hours after AP induction. Rats were killed 24 hours after AP induction. The pancreatic injury was assessed using biochemical and histological approaches. Autophagosomes in pancreatic acinar cells were observed by electron microscopy. The expression levels of microtubule-associated protein 1 light chain 3 (LC3) B/A, beclin-1, and p62/SQSTM1 (p62) were detected by Western blotting, quantitative real-time polymerase chain reaction, and immunohistochemistry in pancreatic tissues.
Compared with non-emodin-treated rats, the pathological injuries of the pancreas of emodin-treated rats were significantly alleviated, and autophagy vacuole formation was reduced within pancreatic acinar cells. Administration of emodin led to a reduction in the autophagy-associated protein level of LC3 (B/A) and p62 but not beclin-1. The transcript levels of LC3B, beclin-1, and p62 were decreased in the emodin-treated rats compared with non-emodin-treated rats.
Our data demonstrate that emodin plays a critical role in ameliorating AP, possibly by down-regulating autophagic protein levels.
本研究旨在探讨大黄素对减轻急性胰腺炎（AP）模型自噬反应的影响。
通过向Wistar大鼠胆胰管注射3%牛磺胆酸钠诱导急性胰腺炎。然后在诱导AP 2小时后给予大黄素（每天40mg/kg）灌胃。在诱导AP 24小时后处死大鼠。采用生化和组织学方法评估胰腺损伤。通过电子显微镜观察胰腺腺泡细胞中的自噬体。采用蛋白质免疫印迹法、定量实时聚合酶链反应和免疫组织化学法检测胰腺组织中微管相关蛋白1轻链3（LC3）B/A、贝林1和p62/ sequestosome 1（p62）的表达水平。
与未用大黄素处理的大鼠相比，用大黄素处理的大鼠胰腺病理损伤明显减轻，胰腺腺泡细胞内自噬泡形成减少。给予大黄素导致LC3（B/A）和p62自噬相关蛋白水平降低，但贝林1未降低。与未用大黄素处理的大鼠相比，用大黄素处理的大鼠中LC3B、贝林1和p62的转录水平降低。
我们的数据表明，大黄素在改善急性胰腺炎中起关键作用，可能是通过下调自噬蛋白水平实现的。
