Lee Hak-Jyung, Yang Ho Yeon, Choi Jong-Il
Department of Biotechnology and Bioengineering, Interdisciplinary Program for Bioenergy and Biomaterials, Chonnam National University,Gwangju 61186, Republic of Korea.
Microbial Institute for Fermentation Industry, Sunchang 56048, Republic of Korea.
J Microbiol Biotechnol. 2018 Jul 28;28(7):1217-1224. doi: 10.4014/jmb.1802.02024.
Seaweed produce antioxidants to counteract environmental stresses, and these antioxidant genes are regarded as important defense strategies for marine algae. In this study, the expression of (Bangiales, Rhodophyta) ascorbate peroxidase () and manganese-superoxide dismutase () was examined by qRT-PCR in blades under abiotic stress conditions. Furthermore, the functional relevance of these genes was explored by overexpressing them in . A comparison of the different expression levels of and after exposure to each stress revealed that both genes were induced by high salt and UVB exposure, being increased approximately 3-fold after 12 h. The expression of the and genes also increased following exposure to HO. When these two genes were overexpressed in , the cells had a higher growth rate than control cells under conditions of hydrogen peroxide-induced oxidative stress, increased salinity, and UV exposure. These data suggest that is a suitable model for studying the function of stress genes, and that and genes are involved in the adaptation and defense against stresses that alter metabolism.
海藻产生抗氧化剂以应对环境压力，这些抗氧化基因被视为海藻的重要防御策略。在本研究中，通过qRT-PCR检测了在非生物胁迫条件下红藻纲（Rhodophyta）角叉菜目（Bangiales）的抗坏血酸过氧化物酶（APX）和锰超氧化物歧化酶（Mn-SOD）在条斑紫菜叶片中的表达。此外，通过在条斑紫菜中过表达这些基因来探究它们的功能相关性。比较暴露于每种胁迫后APX和Mn-SOD的不同表达水平发现，这两个基因均受高盐和UVB暴露诱导，在12小时后增加了约3倍。暴露于H₂O₂后，APX和Mn-SOD基因的表达也增加。当这两个基因在条斑紫菜中过表达时，在过氧化氢诱导的氧化胁迫、盐度增加和紫外线暴露条件下，细胞的生长速率高于对照细胞。这些数据表明，条斑紫菜是研究胁迫基因功能的合适模型，并且APX和Mn-SOD基因参与了对改变代谢的胁迫的适应和防御。
