Kim Jeong Hyeon, Park Eun-Jeong, Choi Jong-Il
Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju, 61186, Republic of Korea.
Aquatic Plant Variety Center, National Institute of Fisheries Science, Mokpo, 58746, Republic of Korea.
Arch Microbiol. 2023 Apr 3;205(5):163. doi: 10.1007/s00203-023-03507-x.
Lipid accumulation in microalgae can be substantially enhanced by exposing the microalgae to abiotic stress, thus increasing biofuel production. However, this also generates reactive oxygen species (ROS), which disrupts cell metabolism and reduces their productivity. Previous mRNA sequencing analyses in Neopyropia yezoensis and its associated microorganisms elucidated a putative glutathione peroxidase (PuGPx) gene. Here, this putative glutathione peroxidase was overexpressed in the microalga Chlamydomonas reinhardtii, which increased cell growth and survival rates compared to the control group under abiotic stress. Additionally, increased lipid accumulation was observed under salinity stress, high-temperature stress, and hydrogen peroxide (HO)-induced oxidative stress. These results suggest that PuGPx plays a protective role against abiotic stress in C. reinhardtii and stimulates lipid accumulation, which could be considered advantageous in terms of biofuel production.
通过使微藻暴露于非生物胁迫下，微藻中的脂质积累可得到显著增强，从而提高生物燃料产量。然而，这也会产生活性氧（ROS），其会扰乱细胞代谢并降低它们的生产力。先前对条斑紫菜及其相关微生物进行的mRNA测序分析阐明了一个假定的谷胱甘肽过氧化物酶（PuGPx）基因。在此，这种假定的谷胱甘肽过氧化物酶在莱茵衣藻中过表达，与非生物胁迫下的对照组相比，这提高了细胞生长和存活率。此外，在盐度胁迫、高温胁迫和过氧化氢（H₂O₂）诱导的氧化胁迫下观察到脂质积累增加。这些结果表明，PuGPx在莱茵衣藻中对非生物胁迫起到保护作用，并刺激脂质积累，就生物燃料生产而言，这可被认为是有利的。
