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条斑紫菜（Pyropia yezoensis）（红毛菜纲，红藻门）HSP70 基因超家族的全基因组分析：鉴定、特征分析及对脱水胁迫的表达谱。

Genome-wide analysis of HSP70 gene superfamily in Pyropia yezoensis (Bangiales, Rhodophyta): identification, characterization and expression profiles in response to dehydration stress.

机构信息

Key Laboratory of Marine Genetics and Breeding (Ministry of Education), Ocean University of China, Qingdao, 266003, China.

College of Marine Life Sciences , Ocean University of China, 5 Yushan Road, Qingdao, 266003, China.

出版信息

BMC Plant Biol. 2021 Sep 24;21(1):435. doi: 10.1186/s12870-021-03213-0.

DOI:10.1186/s12870-021-03213-0
PMID:34560838
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8464122/
Abstract

BACKGROUND

Heat shock proteins (HSPs) perform a fundamental role in protecting plants against abiotic stresses. Individual family members have been analyzed in previous studies, but there has not yet been a comprehensive analysis of the HSP70 gene family in Pyropia yezoensis.

RESULTS

We investigated 15 putative HSP70 genes in Py. yezoensis. These genes were classified into two sub-families, denoted as DnaK and Hsp110. In each sub-family, there was relative conservation of the gene structure and motif. Synteny-based analysis indicated that seven and three PyyHSP70 genes were orthologous to HSP70 genes in Pyropia haitanensis and Porphyra umbilicalis, respectively. Most PyyHSP70s showed up-regulated expression under different degrees of dehydration stress. PyyHSP70-1 and PyyHSP70-3 were expressed in higher degrees compared with other PyyHSP70s in dehydration treatments, and then expression degrees somewhat decreased in rehydration treatment. Subcellular localization showed PyyHSP70-1-GFP and PyyHSP70-3-GFP were in the cytoplasm and nucleus/cytoplasm, respectively. Similar expression patterns of paired orthologs in Py. yezoensis and Py. haitanensis suggest important roles for HSP70s in intertidal environmental adaptation during evolution.

CONCLUSIONS

These findings provide insight into the evolution and modification of the PyyHSP70 gene family and will help to determine the functions of the HSP70 genes in Py. yezoensis growth and development.

摘要

背景

热休克蛋白（HSPs）在保护植物免受非生物胁迫方面发挥着重要作用。在之前的研究中已经分析了个别家族成员，但尚未对条斑紫菜 Pyropia yezoensis 中的 HSP70 基因家族进行全面分析。

结果

我们研究了 Py. yezoensis 中的 15 个假定 HSP70 基因。这些基因分为 DnaK 和 Hsp110 两个亚家族，每个亚家族的基因结构和基序相对保守。基于同线性的分析表明，七个和三个 PyyHSP70 基因分别与 Pyropia haitanensis 和 Porphyra umbilicalis 的 HSP70 基因同源。大多数 PyyHSP70 在不同程度的脱水胁迫下表现出上调表达。与其他 PyyHSP70 相比，PyyHSP70-1 和 PyyHSP70-3 在脱水处理中表达程度更高，然后在复水处理中表达程度略有下降。亚细胞定位显示 PyyHSP70-1-GFP 和 PyyHSP70-3-GFP 分别位于细胞质和核/细胞质中。Py. yezoensis 和 Py. haitanensis 中配对同源物的相似表达模式表明 HSP70 在进化过程中对潮间带环境适应具有重要作用。

结论

这些发现为 PyyHSP70 基因家族的进化和修饰提供了深入了解，并有助于确定 HSP70 基因在 Py. yezoensis 生长和发育中的功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58c9/8464122/8f2741255e4a/12870_2021_3213_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58c9/8464122/3aed286dc812/12870_2021_3213_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58c9/8464122/7e35cefb7a5c/12870_2021_3213_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58c9/8464122/94a43fffdf57/12870_2021_3213_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58c9/8464122/c5bc869e6963/12870_2021_3213_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58c9/8464122/814050b2da4e/12870_2021_3213_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58c9/8464122/8f2741255e4a/12870_2021_3213_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58c9/8464122/3aed286dc812/12870_2021_3213_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58c9/8464122/7e35cefb7a5c/12870_2021_3213_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58c9/8464122/94a43fffdf57/12870_2021_3213_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58c9/8464122/c5bc869e6963/12870_2021_3213_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58c9/8464122/814050b2da4e/12870_2021_3213_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58c9/8464122/8f2741255e4a/12870_2021_3213_Fig6_HTML.jpg

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