研究主要臭氧自血疗法应用对红细胞变形性和聚集的影响。

Investigation of the effects of major ozone autohemotherapy application on erythrocyte deformability and aggregation.

机构信息

Department of Internal Medicine, Faculty of Medicine, Pamukkale University, Denizli, Turkey.

Department of Physiology, Faculty of Medicine, Pamukkale University, Denizli, Turkey.

出版信息

Clin Hemorheol Microcirc. 2019;71(3):365-372. doi: 10.3233/CH-180417.

DOI:10.3233/CH-180417

PMID:29914015

Abstract

BACKGROUND

Ozone is used intensively worldwide in treatment and research of various pathologies due to its healing effects.

OBJECTIVE

The aim of this study is to investigate the effect of major ozone autohemotherapy on erythrocyte deformability and aggregation.

METHODS

10 and 50μg/ml doses of ozone was applied for 20 minute to venous blood samples obtained from 10 healthy male volunteers. Erythrocyte aggregation, deformability were measured by an ektacytometer. Total oxidant status, total antioxidant status were measured via a commercial kit. The oxidative stress index was calculated.

RESULTS

Ozone at 10 and 50μg/ml doses did not alter erythrocyte aggregation. 50μg/ml ozone increased red blood cell (RBC) deformability measured at 0.53 Pa. Compared with the Control value, there was a significant increase in TOS, TAS for the doses of 10 and 50μg/ml. The increase in TAS was found to be more significant at 10μg/ml dose. The most obvious increase in OSI value was observed at 50μg/ml.

CONCLUSION

Our results demonstrate that although 10μg/ml ozone has no effect on hemorheology, 50μg/ml ozone concentration has positive effects on RBC deformability, thus circulation at 0.53 Pa corresponding to the shear stress encountered during venous circulation.

摘要

背景

由于臭氧具有治疗作用，因此在世界范围内被广泛用于治疗和研究各种疾病。

目的

本研究旨在探讨主要臭氧自体血疗法对红细胞变形性和聚集性的影响。

方法

将 10μg/ml 和 50μg/ml 剂量的臭氧应用于 10 名健康男性志愿者的静脉血样本 20 分钟。通过 ektacytometer 测量红细胞聚集性和变形性。通过商业试剂盒测量总氧化剂状态和总抗氧化状态。计算氧化应激指数。

结果

10μg/ml 和 50μg/ml 剂量的臭氧不会改变红细胞聚集性。50μg/ml 臭氧增加了在 0.53 Pa 时测量的红细胞（RBC）变形性。与对照值相比，10μg/ml 和 50μg/ml 剂量的 TOS 和 TAS 均显著增加。在 10μg/ml 剂量下，TAS 的增加更为明显。在 50μg/ml 时观察到 OSI 值的最明显增加。