Küçük Ayşegül, Köksal Zeynep, Yeşil Süleyman, Şengel Necmiye, Sezen Şaban Cem, Güneş Işın, Atlı Muharrem, Kip Gülay, Acar Seda Gökgöz, Özer Abdullah, Kavutçu Mustafa, Arslan Mustafa
Department of Physiology, Kütahya Health Sciences University Faculty of Medicine, Kütahya, Turkey.
Department Anesthesiology and Reanimation, Ankara 29 Mayıs State Hospital, Ankara, Turkey.
Drug Des Devel Ther. 2025 Aug 18;19:7089-7098. doi: 10.2147/DDDT.S528786. eCollection 2025.
Spinal cord ischemia-reperfusion injury (IRI) is a serious condition that can develop after spinal and thoracoabdominal surgeries or spinal cord traumas. The aim of this study was to investigate the effects of different administration routes of ozone on testicular tissue in rats with spinal cord ischemia and reperfusion injury.
Rats were divided into five groups (n:5): control (C), ischemia-reperfusion (IR), IR-rectal ozone (IR+RO), IR-intratechal ozone (IR+ITO), and IR-intraperitoneal (i.p) ozone (IR+IPO). Ozone-oxygen mixture was administered 30 minutes before midline laparotomy: 1 mg/kg (50 µg/mL) by rectal insufflation to the IR+RO group, 20 µL (20 µg/mL) intratechally to the IR+ITO group, and 0.7 µg/kg (50 µg/mL) intraperitoneally to the IR+IPO group. The spinal cord IR model was established. The testicular tissue was collected for histopathological and biochemical analyses.
The Malondialdehyde (MDA) levels and Paraoxonase-1 (PON1) activities were significantly lower in the IR+RO, IR+ITO, and IR+IPO groups than in the IR group. Catalase (CAT) was significantly higher in the IR+ITO and IR+IPO groups than in the IR group. While the Cosentino score was significantly higher in the IR group than in the C group (p<0.001), it was significantly lower in the IR+RO, IR+ITO and IR+IPO groups than in the IR group (p<0.001, all).
Ozone can regulate the negative effects of IRI by regulating cellular oxidative stress mechanisms. This effect can be achieved most effectively on testis tissue in the spinal cord IRI model through intrathecal and intraperitoneal administration.
脊髓缺血再灌注损伤（IRI）是一种严重的病症，可发生于脊柱和胸腹手术或脊髓创伤后。本研究的目的是探讨不同给药途径的臭氧对脊髓缺血再灌注损伤大鼠睾丸组织的影响。
将大鼠分为五组（每组n = 5）：对照组（C）、缺血再灌注组（IR）、IR-直肠臭氧组（IR+RO）、IR-鞘内臭氧组（IR+ITO）和IR-腹腔内（i.p）臭氧组（IR+IPO）。在中线剖腹术前30分钟给予臭氧-氧气混合物：IR+RO组通过直肠注入1mg/kg（50μg/mL），IR+ITO组鞘内注射20μL（20μg/mL），IR+IPO组腹腔内注射0.7μg/kg（50μg/mL）。建立脊髓IR模型。收集睾丸组织进行组织病理学和生化分析。
IR+RO组、IR+ITO组和IR+IPO组的丙二醛（MDA）水平和对氧磷酶-1（PON1）活性显著低于IR组。IR+ITO组和IR+IPO组的过氧化氢酶（CAT）显著高于IR组。虽然IR组的Cosentino评分显著高于C组（p<0.001），但IR+RO组、IR+ITO组和IR+IPO组的Cosentino评分显著低于IR组（均为p<0.001）。
臭氧可通过调节细胞氧化应激机制来调节IRI的负面影响。在脊髓IRI模型中，通过鞘内和腹腔内给药对睾丸组织可最有效地实现这种效果。
