Capecchi Eliana, Piccinino Davide, Delfino Ines, Bollella Paolo, Antiochia Riccarda, Saladino Raffaele
Department of Biological and Ecological Sciences, University of Tuscia, Via S. Camillo de Lellis snc, 01100 Viterbo, Italy.
Department of Chemistry and Drug Technologies, Sapienza University of Rome P.le Aldo Moro 5, 00185 Rome, Italy.
Nanomaterials (Basel). 2018 Jun 15;8(6):438. doi: 10.3390/nano8060438.
Sustainable catalysts for the oxidation of phenol derivatives under environmentally friendly conditions were prepared by the functionalization of lignin nanoparticles with tyrosinase. Lignin, the most abundant polyphenol in nature, is the main byproduct in the pulp and paper manufacturing industry and biorefinery. Tyrosinase has been immobilized by direct adsorption, encapsulation, and layer-by-layer deposition, with or without glutaraldehyde reticulation. Lignin nanoparticles were found to be stable to the tyrosinase activity. After the enzyme immobilization, they showed a moderate to high catalytic effect in the synthesis of catechol derivatives, with the efficacy of the catalyst being dependent on the specific immobilization procedures.
通过用酪氨酸酶对木质素纳米颗粒进行功能化处理，制备了在环境友好条件下用于氧化苯酚衍生物的可持续催化剂。木质素是自然界中最丰富的多酚，是制浆造纸工业和生物精炼中的主要副产物。酪氨酸酶已通过直接吸附、包封和层层沉积进行固定，有无戊二醛交联均可。发现木质素纳米颗粒对酪氨酸酶活性具有稳定性。酶固定后，它们在儿茶酚衍生物的合成中表现出中度至高催化效果，催化剂的功效取决于特定的固定程序。
