State Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, No. 29 The Thirteenth Road, Tianjin Economy and Technology Development Area, Tianjin 300457, China.
Sensors (Basel). 2018 Jun 15;18(6):1946. doi: 10.3390/s18061946.
In this work, a sensitive electrochemical immunosensor has been reported for the determination of norfloxacin in animal-derived foods. The poly (amidoamine) dendrimer encapsulated gold nanoparticles (PAMAM-Au) played dual roles in the proposed sensing platform which not only accelerated the electron transfer process of sensing, but also increased the efficiency of the immobilized antibody. The HRP-labeled antigen, as the signal labels in the immunosensor, was introduced to catalyze the following reaction of the substrate hydroquinone with the aid of H₂O₂ in the competitive reaction. On the basis of the signal amplification of PAMAM-Au, the signal intensity was linearly related to the concentration of norfloxacin in the range of 1 μg·L⁻10 mg·L. All the results showed that the proposed strategy with low LOD (0.3837 μg·L) and favorable recovery (91.6⁻106.1%) in the practical sample, and it could provide a suitable protocol for norfloxacin detection in animal-derived foods with high sensitivity, good accuracy, and stability.
在这项工作中，我们报道了一种用于测定动物源性食品中诺氟沙星的敏感电化学免疫传感器。多聚（酰胺-胺）树状大分子包裹的金纳米粒子（PAMAM-Au）在提出的传感平台中发挥了双重作用，不仅加速了传感过程中的电子转移过程，而且提高了固定化抗体的效率。辣根过氧化物酶标记的抗原作为免疫传感器中的信号标记物，在竞争反应中在 H₂O₂的辅助下，被引入以催化底物对苯二酚的反应。基于 PAMAM-Au 的信号放大，信号强度与诺氟沙星浓度在 1 μg·L-10 mg·L 的范围内呈线性关系。所有结果表明，该方法具有低检测限（0.3837 μg·L）和良好的回收率（91.6-106.1%），可用于动物源性食品中诺氟沙星的检测，具有灵敏度高、准确性好、稳定性好等优点。
