枯草芽孢杆菌噬菌体SPP1的基因组：一个早期启动子的结构

The genome of Bacillus subtilis phage SPP1: structure of an early promoter.

作者信息

Tailor R, Bensi G, Morelli G, Canosi U, Trautner T A

出版信息

J Gen Microbiol. 1985 May;131(5):1259-62. doi: 10.1099/00221287-131-5-1259.

Abstract

The strongest of five 'early' promoters of Bacillus subtilis phage SPP1 was localized in a DNA restriction fragment by analysis of RNA polymerase binding and R-loop formation. The nucleotide sequence of the promoter region was established. The signal structures identified were similar to those recognized by the sigma 55 RNA polymerase of B. subtilis. The promoter precedes an open reading frame with 51 codons. A protein with the Mr predicted from the nucleotide sequence was identified in minicells.

摘要

通过对RNA聚合酶结合和R环形成的分析，枯草芽孢杆菌噬菌体SPP1五个“早期”启动子中最强的一个被定位在一个DNA限制片段中。确定了启动子区域的核苷酸序列。所识别的信号结构与枯草芽孢杆菌的σ55 RNA聚合酶识别的结构相似。该启动子位于一个含有51个密码子的开放阅读框之前。在微型细胞中鉴定出了一种具有根据核苷酸序列预测的分子量的蛋白质。