Lee G, Talkington C, Pero J
Mol Gen Genet. 1980;180(1):57-65. doi: 10.1007/BF00267352.
We report the nucleotide sequence of a promoter recognized by RNA polymerase from the gram-positive bacterium Bacillus subtilis. This promoter, which was isolated from B. subtilis phage SP01 DNA, is homologous to promoters for Escherichia coli RNA polymerase; the sequences of the "-35 region" and the "Pribnow box" were 5'TTGACT and 5'CATAAT, respectively (T is the thymine analog 5-hydroxymethyluracil in SP01 DNA). These sequences each differed by only a single base pair from the preferred sequences for E. coli promoters. Not surprisingly, the SP01 promoter was actively transcribed in vitro by E. coli RNA Polymerase as well as by B. subtilis RNA polymerase.
我们报道了一种由革兰氏阳性细菌枯草芽孢杆菌的RNA聚合酶识别的启动子的核苷酸序列。该启动子从枯草芽孢杆菌噬菌体SP01 DNA中分离得到，与大肠杆菌RNA聚合酶的启动子同源；“-35区”和“Pribnow框”的序列分别为5'TTGACT和5'CATAAT（在SP01 DNA中，T是胸腺嘧啶类似物5-羟甲基尿嘧啶）。这些序列与大肠杆菌启动子的优选序列相比，每个序列仅相差一个碱基对。不出所料，SP01启动子在体外能被大肠杆菌RNA聚合酶以及枯草芽孢杆菌RNA聚合酶积极转录。
