Bhat M A, Gandhi G
a Department of Human Genetics , Guru Nanak Dev University , Amritsar , India.
Acta Cardiol. 2019 Apr;74(2):153-160. doi: 10.1080/00015385.2018.1475093. Epub 2018 Jun 18.
The objective of the present study was to evaluate oxidative DNA damage in peripheral blood leukocytes (PBLs) of patients with coronary artery disease (CAD) and to explore the relationship of oxidised purine and pyrimidine with oxidative stress.
The study participants (n = 100) included 50 patients and unrelated 50 age-, sex- and population-subgroup (Jat Sikhs)-matched healthy controls. Oxidative DNA damage using the modified enzymatic comet in PBLs, and malondialdehyde (MDA) levels, total oxidant status (TOS) and total antioxidant status (TAS) in blood serum samples using spectrophotometric methods was determined.
The basal DNA damage of percent tail DNA (T-DNA%) was increased as were tail moment (TM) and olive tail moment (OTM). Oxidative DNA damage in terms of oxidised purines and oxidised pyrimidines was also significantly (p < .001) elevated in patients. Rather the advanced stages of CAD, unstable angina and acute myocardial infarction had significantly more basal and oxidative DNA damage (p < .05) compared to stable angina. MDA levels (p < .01) and TOS (p < .001) were increased significantly in patients with significant (p < .001) decrease in TAS. There was positive correlation of oxidised purines (T-DNA% r = 0.399, p = .004; TM r = 0.623, p = .001; OTM r = 0.456, p= .001) and of total oxidative damage (TM r = 0.515, p = .001; OTM r = 0.463, p = .001) with disease severity, and, with TOS (r = 0.279, p = .050) and negative with TAS (r = -0.341, p = .015). Multiple linear regression analysis revealed TOS and disease severity as independent predictors of oxidative DNA damage.
There was significant increase in oxidative DNA damage and oxidative stress in CAD patients compared to levels in healthy controls.
本研究旨在评估冠心病（CAD）患者外周血白细胞（PBLs）中的氧化性DNA损伤，并探讨氧化嘌呤和嘧啶与氧化应激的关系。
研究参与者（n = 100）包括50例患者和50例年龄、性别及人群亚组（贾特锡克人）匹配的无血缘关系健康对照。采用改良酶促彗星试验测定PBLs中的氧化性DNA损伤，采用分光光度法测定血清样本中的丙二醛（MDA）水平、总氧化剂状态（TOS）和总抗氧化剂状态（TAS）。
尾DNA百分比（T-DNA%）、尾矩（TM）和橄榄尾矩（OTM）等基础DNA损伤增加。患者中氧化嘌呤和氧化嘧啶方面的氧化性DNA损伤也显著（p <.001）升高。与稳定型心绞痛相比，CAD的晚期阶段、不稳定型心绞痛和急性心肌梗死的基础和氧化性DNA损伤显著更多（p <.05）。患者的MDA水平（p <.01）和TOS（p <.001）显著升高，而TAS显著降低（p <.001）。氧化嘌呤（T-DNA% r = 0.399，p =.004；TM r = 0.623，p =.001；OTM r = 0.456，p =.001）和总氧化损伤（TM r = 0.515，p =.001；OTM r = 0.463，p =.001）与疾病严重程度呈正相关，与TOS呈正相关（r = 0.279，p =.050），与TAS呈负相关（r = -0.341，p =.015）。多元线性回归分析显示TOS和疾病严重程度是氧化性DNA损伤的独立预测因素。
与健康对照相比，CAD患者的氧化性DNA损伤和氧化应激显著增加。
