Department of Biology and Ecology, Faculty of Science, University of Kragujevac; Kragujevac-Serbia.
Department of Internal Medicine, Faculty of Medical Sciences, University of Kragujevac; Kragujevac-Serbia;Department of Cardiology, Clinical Center Kragujevac; Kragujevac-Serbia.
Anatol J Cardiol. 2021 Apr;25(4):243-249. doi: 10.14744/AnatolJCardiol.2020.39479.
The aim of the study was to evaluate the DNA and chromosomal damage in peripheral blood lymphocytes (PBLs) of patients with acute coronary syndrome (ACS) and to explore the effect of coronary angiographies in these patients.
The study included ACS patients who underwent a coronary angiography (CAG) and healthy controls. The ACS sample was divided into two groups: patients with unstable angina pectoris (UAP) and acute myocardial infarction (AMI). The frequency of DNA damage [expressed as genetic damage index (GDI)] was analyzed using the comet assay pre- and post-CAG. Chromosomal aberrations were measured as micronuclei (MNs) frequency using the cytokinesis-block MN (CBMN) assay. Additionally, detailed anamnestic data were taken from the each patient.
Increased levels of DNA and chromosomal damage have been revealed in ACS patients compared to the healthy controls. GDI values were also significantly higher in AMI patients than in UAP patients. A highly significant increase of DNA damage was also observed in all patients post-CAG. There was significantly higher MN frequency and significantly lower nuclear division index (NDI) in AMI patients than in UAP patients' pre-CAG. After CAG, there was no significant difference in MN frequencies and NDI values between UAP and AMI patients.
Correlated with disease severity, our results showed that AMI patients have higher levels of both DNA and chromosomal damage in PBLs compared to UAP patients. The increased level of genome instability was especially evident post-CAG compared to the observed damage pre-CAG.
本研究旨在评估急性冠状动脉综合征（ACS）患者外周血淋巴细胞（PBL）中的 DNA 和染色体损伤，并探讨冠状动脉造影（CAG）对这些患者的影响。
本研究纳入了接受 CAG 的 ACS 患者和健康对照者。ACS 样本分为不稳定型心绞痛（UAP）和急性心肌梗死（AMI）两组。采用彗星试验分析 DNA 损伤频率（以遗传损伤指数[GDI]表示），并在 CAG 前后进行分析。采用胞质分裂阻断微核（CBMN）试验测量染色体畸变作为微核（MN）频率。此外，还从每位患者那里获取了详细的病史数据。
与健康对照组相比，ACS 患者的 DNA 和染色体损伤水平升高。AMI 患者的 GDI 值也明显高于 UAP 患者。所有患者 CAG 后 DNA 损伤也明显增加。与 UAP 患者 CAG 前相比，AMI 患者的 MN 频率更高，核分裂指数（NDI）更低。CAG 后，UAP 和 AMI 患者的 MN 频率和 NDI 值无显著差异。
与疾病严重程度相关，我们的结果表明，与 UAP 患者相比，AMI 患者 PBL 中的 DNA 和染色体损伤水平更高。与 CAG 前观察到的损伤相比，CAG 后基因组不稳定性水平增加更为明显。
