Matthews S B, Henderson A H, Campbell A K
J Mol Cell Cardiol. 1985 Apr;17(4):339-48. doi: 10.1016/s0022-2828(85)80133-4.
Oxygen radical production by polymorphonuclear leucocytes stimulated the oxidation of adrenalin through the adrenochrome pathway. This was detected either spectrophotometrically at 480 nm or separated by hplc and detected radiochemically. The oxidation was detectable within 5 min and continued for at least 4 h. Over the adrenalin concentration range 0.3 microM to 10 mM more than 80% of the oxidation that occurred was through the adrenochrome pathway, the remainder being through the amine oxidase, catechol methyl transferase pathway. Medium isolated after stimulation of the polymorphonuclear leucocytes was also able to oxidise adrenalin to adrenochrome. The results provide a cellular mechanism for the formation of adrenochrome and the other metabolites on this pathway of adrenalin metabolism, in inflammatory conditions where polymorphonuclear leucocyte infiltration occurs.
多形核白细胞产生的氧自由基通过肾上腺色素途径刺激肾上腺素氧化。这可以通过在480nm处进行分光光度检测，或者通过高效液相色谱分离并进行放射化学检测。氧化在5分钟内即可检测到，并持续至少4小时。在肾上腺素浓度范围为0.3微摩尔至10毫摩尔时，超过80%的氧化反应是通过肾上腺色素途径发生的，其余部分则通过胺氧化酶、儿茶酚甲基转移酶途径。多形核白细胞受到刺激后分离出的培养基也能够将肾上腺素氧化为肾上腺色素。这些结果为在发生多形核白细胞浸润的炎症条件下，肾上腺色素和肾上腺素代谢这条途径上的其他代谢产物的形成提供了一种细胞机制。
