Skare J, Farley J, Strominger J L, Fresen K O, Cho M S, zur Hausen H
J Virol. 1985 Aug;55(2):286-97. doi: 10.1128/JVI.55.2.286-297.1985.
Eight independent recombinant Epstein-Barr virus genomes, each of which was a transforming strain, were made by superinfecting cell lines containing Epstein-Barr virus DNA (Raji or B95-8 strain) with a nontransforming virus (P3HR1 strain). A knowledge of the constitution of each transforming recombinant allowed the localization of the defect in the genome of the nontransforming parent to a 12-megadalton sequence within the EcoRI A fragment. Within this region, the nontransforming virus has a deletion of the BamHI Y fragment and about half of the sequences in the adjacent BamHI H fragment. The present data suggest that this deletion is responsible for the nontransforming phenotype. Furthermore, mapping a deletion in one of the recombinant genomes allowed the conclusion that a sequence (comprising about 20% of the Epstein-Barr virus genome) from the center of BamHI-D to BamHI-I' is not necessary for the maintenance of transformation by Epstein-Barr virus.
通过用非转化病毒（P3HR1株）对含有爱泼斯坦 - 巴尔病毒DNA的细胞系（Raji或B95 - 8株）进行超感染，制备了八个独立的重组爱泼斯坦 - 巴尔病毒基因组，每个基因组都是转化株。了解每个转化重组体的组成后，可将非转化亲本基因组中的缺陷定位到EcoRI A片段内的一个12兆道尔顿序列上。在该区域内，非转化病毒缺失了BamHI Y片段以及相邻BamHI H片段中约一半的序列。目前的数据表明，这种缺失导致了非转化表型。此外，对其中一个重组基因组中的缺失进行定位后得出结论，从BamHI - D中心到BamHI - I'的一段序列（约占爱泼斯坦 - 巴尔病毒基因组的20%）对于爱泼斯坦 - 巴尔病毒维持转化并非必需。
