Grogan E A, Summers W P, Dowling S, Shedd D, Gradoville L, Miller G
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7650-3. doi: 10.1073/pnas.80.24.7650.
We recently identified, by means of cotransformation of LTK- cells, a region of the Epstein-Barr virus (EBV) genome (the BamHI K fragment) that encodes or induces an EBV nuclear neoantigen (EBNA) serologically related to the EBNA found in lymphoid cells carrying the entire EBV genome. We now find that a second EBV DNA fragment, BamHI M, is also able to give rise to cotransformed LTK- cells with stable expression of a nuclear antigen. The BamHI K and M fragments have no apparent DNA homology. Many human sera that are reactive to EBNA in Raji cells detect both antigens; however, certain anti-EBNA-positive human sera are discordant and react only with the BamHI M or only with the BamHI K nuclear antigen. Every Raji cell appears to express both "M" and "K" antigens; D98 Raji cells, a somatic cell hybrid, express only "K" antigen. The K antigen is found on metaphase chromosomes of LTK cells and Raji cells. The M-induced antigen is not located on chromosomes when the cells are in metaphase but is present as granules within the nucleus.
我们最近通过对LTK⁻细胞进行共转化，鉴定出爱泼斯坦-巴尔病毒（EBV）基因组的一个区域（BamHI K片段），该区域编码或诱导一种EBV核新抗原（EBNA），在血清学上与在携带完整EBV基因组的淋巴细胞中发现的EBNA相关。我们现在发现，另一个EBV DNA片段BamHI M也能够产生共转化的LTK⁻细胞，并稳定表达一种核抗原。BamHI K和M片段没有明显的DNA同源性。许多对Raji细胞中的EBNA有反应的人血清能检测到这两种抗原；然而，某些抗EBNA阳性的人血清不一致，只与BamHI M或只与BamHI K核抗原发生反应。每个Raji细胞似乎都表达“M”和“K”抗原；体细胞杂种D98 Raji细胞只表达“K”抗原。K抗原存在于LTK细胞和Raji细胞的中期染色体上。当细胞处于中期时，M诱导的抗原不在染色体上，而是以颗粒形式存在于细胞核内。
