Chemistry Department, Sharif University of Technology, Tehran, 11155-9516, Iran.
Chemistry and Chemical Engineering Research Center of Iran, 14335-186, Tehran, Iran.
Analyst. 2018 Jul 21;143(14):3415-3424. doi: 10.1039/c8an00637g. Epub 2018 Jun 19.
Considering the crucial role of biothiols in many biological processes, which turns them into highly valuable biomarkers for the early diagnosis of various diseases, the development of an affordable, sensitive and portable probe for the identification and discrimination of these compounds is of great importance. Herein, we developed a ratiometric fluorescent (RF) sensor array with a wide color emissive variation, on a bacterial cellulose (BC) nanopaper substrate for the visual discrimination of biothiols. To this aim, RF sensing elements including N-acetyl l-cysteine capped green CdTe quantum dots-rhodamine B (GQDs-RhB) and red CdTe QDs-carbon dots (RQDs-CDs) at two different NaOH concentrations (0 and 5 mM) were utilized as sensor elements for the discrimination of biothiols. Owing to the high affinity of the thiol group (SH) to the surface of CdTe QDs and the aggregation of the QDs, the fluorescence (FL) emission of the QDs changed while the emission of the CDs and rhodamine B remained almost unchanged upon the addition of biothiols. Accordingly, characteristic rainbow-like FL fingerprint patterns were created for each biothiol which were then distinguished both visually and spectroscopically. Hierarchical cluster analysis (HCA) and linear discriminant analysis (LDA) pattern recognition techniques were employed for the identification and discrimination of biothiols. Based on the designed RF sensor array, convenient test strips were fabricated on BC nanopaper for the visual discrimination of biothiols. It has been shown that this probe can successfully identify biothiols in human plasma as well. Altogether, the developed nanopaper-based sensor array offers an efficient biothiol discrimination tool that can be potentially exploited in the near future in theranostic and point-of-care applications.
考虑到生物硫醇在许多生物过程中的关键作用，它们成为各种疾病早期诊断的极具价值的生物标志物，因此开发一种经济实惠、灵敏且便携的探针来识别和区分这些化合物非常重要。在此，我们在细菌纤维素（BC）纳米纸上开发了一种具有宽发射颜色变化的比率荧光（RF）传感器阵列，用于生物硫醇的可视化区分。为此，使用了包括 N-乙酰-L-半胱氨酸封端的绿色 CdTe 量子点-罗丹明 B（GQDs-RhB）和在两种不同 NaOH 浓度（0 和 5 mM）下的红色 CdTe QDs-碳点（RQDs-CDs）在内的 RF 传感元件作为传感器元件，用于区分生物硫醇。由于巯基（SH）与 CdTe QDs 表面的高亲和力以及 QDs 的聚集，当加入生物硫醇时，QDs 的荧光（FL）发射会发生变化，而 CDs 和罗丹明 B 的发射几乎保持不变。因此，为每种生物硫醇创建了特征性的彩虹状 FL 指纹图谱，然后可以通过视觉和光谱来区分它们。采用层次聚类分析（HCA）和线性判别分析（LDA）模式识别技术对生物硫醇进行识别和区分。基于设计的 RF 传感器阵列，在 BC 纳米纸上制备了方便的测试条，用于生物硫醇的可视化区分。结果表明，该探针可以成功识别人血浆中的生物硫醇。总之，所开发的基于纳米纸的传感器阵列提供了一种有效的生物硫醇区分工具，有望在不久的将来在治疗和即时护理应用中得到应用。
