Radzun H J, Parwaresch M R, Bödewadt S, Sundström C, Lennert K
J Natl Cancer Inst. 1985 Aug;75(2):199-206.
In accordance with the long-range goal to demonstrate the direct derivation of blood monocytes from promyelocytes in human bone marrow, the promyelocytic cell line HL-60 in the present study was differently stimulated with various inducers for the purpose of documenting its capability to evolve into granulocytes or monocytes-macrophages. Each differentiation line was monitored with the use of marker enzymes, antigens, and cell-specific monoclonal antibodies. In addition, studies were done on normal human bone marrow and leukemias. The results provided strong evidence for the close cytogeneic relationship of granulocytes and monocytes and for a common progenitor at the maturation stage of promyelocytes-myelocytes for both cell lineages.
为了证明人类骨髓中早幼粒细胞可直接分化为血液单核细胞这一长期目标，本研究中使用不同诱导剂对早幼粒细胞系HL - 60进行不同刺激，以记录其向粒细胞或单核细胞 - 巨噬细胞分化的能力。每个分化系都通过标记酶、抗原和细胞特异性单克隆抗体进行监测。此外，还对正常人类骨髓和白血病进行了研究。结果为粒细胞和单核细胞之间密切的细胞发生关系以及两个细胞系在早幼粒细胞 - 中幼粒细胞成熟阶段存在共同祖细胞提供了有力证据。
