Han T, Minowada J
J Clin Lab Immunol. 1980 Nov;4(3):169-73.
The present study describes a comparative study of MLR-S of various stages of fresh and cultured normal or leukemic myeloid, monocytic and erythroid cells. "One-way" MLR was performed, using a slightly modified whole blood method. Fresh leukemic myeloblasts from patients with acute myelocytic leukemia or chronic myelocytic leukemia, in blastic crisis possess a strong MLR-S whereas fresh granulocytes from patients with chronic myelocytic leukemia or from healthy subjects possess no MLR-S. Cultured leukemic myeloblasts from KG-1 or ML-1 cell line possess a very strong or a moderate MLR-S, whereas cultured leukemic promyelocytes from HL-60 cell line possess little or no MLR-S. Fresh leukemic erythroblasts or cultured leukemic erythroblasts from K-562 cell line exert a strong MLR-S whereas fresh erythrocytes exert no MLR-S. Cultured monoblasts from HPL-SK-1 or Murray cell line possess a very strong MLR-S whereas fresh monocytes from healthy subjects or from a patient with chronic monocytic leukemia possess a moderate MLR-S. These observations clearly indicate that there is a good correlation between the MLR-S and the cell differentiation stage. Observations in the present study also support the hypothesis that the MLR-S is a differentiation antigen which is completely lost by the terminal stage of myeloid or erythroid cell maturation or partially lost by the terminal stage of monocytic cell maturation.
本研究描述了对新鲜的和培养的正常或白血病髓系、单核系及红系细胞不同阶段的混合淋巴细胞反应抑制（MLR-S）的比较研究。采用略有改良的全血法进行“单向”混合淋巴细胞反应。急性髓细胞白血病或慢性髓细胞白血病急变期患者的新鲜白血病原始粒细胞具有很强的MLR-S，而慢性髓细胞白血病患者或健康受试者的新鲜粒细胞则无MLR-S。来自KG-1或ML-1细胞系的培养白血病原始粒细胞具有很强或中等强度的MLR-S，而来自HL-60细胞系的培养白血病早幼粒细胞则几乎没有或没有MLR-S。来自K-562细胞系的新鲜白血病有核红细胞或培养白血病有核红细胞具有很强的MLR-S，而新鲜红细胞则无MLR-S。来自HPL-SK-1或Murray细胞系的培养单核细胞具有很强的MLR-S，而健康受试者或慢性单核细胞白血病患者的新鲜单核细胞具有中等强度的MLR-S。这些观察结果清楚地表明，MLR-S与细胞分化阶段之间存在良好的相关性。本研究中的观察结果也支持这样的假说，即MLR-S是一种分化抗原，在髓系或红系细胞成熟末期完全丧失，或在单核细胞成熟末期部分丧失。
