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多瘤病毒突变体F9-1 DNA调控区的功能分析

Functional analysis of the regulatory region of polyoma mutant F9-1 DNA.

作者信息

Böhnlein E, Chowdhury K, Gruss P

出版信息

Nucleic Acids Res. 1985 Jul 11;13(13):4789-809. doi: 10.1093/nar/13.13.4789.

DOI:10.1093/nar/13.13.4789
PMID:2991846
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC321827/
Abstract

Functional analysis of the transcriptional control region of the polyoma (Py) mutant F9-1 reveals that the mutation is located in a region of Py DNA required for at least two functions. First, an enhancer element which includes the F9-1 mutation was characterized by deletion analysis. This element, described previously as enhancer B is essential for viral early gene expression in F9 stem cells whereas enhancer A is unnecessary for transcriptional activity in embryonal carcinoma (EC) cells. Second, a CCACCC motif, present twice in the 3' part of enhancer B is also required in cis for the response to a heterologous enhancer. This suggests that a promoter element is present in this region of the polyoma genome which overlaps Py enhancer B. We also demonstrate the enhancement of the polyoma early promoter activity in F9 stem cells by MSV sequences. The significance of these observations is discussed.

摘要

多瘤病毒（Py）突变体F9-1转录控制区的功能分析表明，该突变位于Py DNA的一个区域，该区域至少具有两种功能。首先，通过缺失分析对包含F9-1突变的增强子元件进行了表征。该元件先前被描述为增强子B，对于F9干细胞中的病毒早期基因表达至关重要，而增强子A对于胚胎癌（EC）细胞中的转录活性则不是必需的。其次，增强子B 3'部分中出现两次的CCACCC基序对于对异源增强子的应答在顺式作用中也是必需的。这表明在多瘤病毒基因组的该区域中存在一个与Py增强子B重叠的启动子元件。我们还证明了MSV序列可增强F9干细胞中多瘤病毒早期启动子的活性。讨论了这些观察结果的意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8232/321827/45210c316bc9/nar00307-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8232/321827/469af5739695/nar00307-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8232/321827/8eec54e651d6/nar00307-0187-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8232/321827/d0e09402e5f5/nar00307-0189-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8232/321827/aff579fa14ee/nar00307-0192-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8232/321827/45210c316bc9/nar00307-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8232/321827/469af5739695/nar00307-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8232/321827/8eec54e651d6/nar00307-0187-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8232/321827/d0e09402e5f5/nar00307-0189-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8232/321827/aff579fa14ee/nar00307-0192-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8232/321827/45210c316bc9/nar00307-0194-a.jpg

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