Leer R J, van Raamsdonk-Duin M M, Molenaar C M, Witsenboer H M, Mager W H, Planta R J
Nucleic Acids Res. 1985 Jul 25;13(14):5027-39. doi: 10.1093/nar/13.14.5027.
The DNA sequence of the second copy of the gene coding for yeast ribosomal protein S10 was determined and compared with the sequence of the first gene-copy. In addition, the sites at which the transcription of these genes start and terminate are identified. The amino acid coding regions of the two gene copies are virtually identical. The leader and in particular the trailer sequences, however, are significantly different, while the intervening sequences have hardly any homology. Taking advantage of the sequence differences we could establish that both genes are expressed in the vegetatively growing yeast cell; the respective transcripts, however, differ in their relative amounts.
测定了编码酵母核糖体蛋白S10的基因第二个拷贝的DNA序列,并将其与第一个基因拷贝的序列进行了比较。此外,还确定了这些基因转录起始和终止的位点。两个基因拷贝的氨基酸编码区几乎相同。然而,前导序列,尤其是尾序列有显著差异,而间隔序列几乎没有任何同源性。利用序列差异,我们可以确定这两个基因在营养生长的酵母细胞中均有表达;然而,各自的转录本在相对含量上有所不同。