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转录因子Sp1识别来自猴基因组的启动子序列，这些序列是猿猴病毒40启动子。

Transcription factor Sp1 recognizes promoter sequences from the monkey genome that are simian virus 40 promoter.

作者信息

Dynan W S, Saffer J D, Lee W S, Tjian R

出版信息

Proc Natl Acad Sci U S A. 1985 Aug;82(15):4915-9. doi: 10.1073/pnas.82.15.4915.

DOI:10.1073/pnas.82.15.4915
PMID:2991898
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC390468/
Abstract

A 440-base-pair fragment of African green monkey genomic DNA shares homology with the transcriptional regulatory region of simian virus 40 (SV40) and has been reported to direct transcription in vivo. We find that two regions within this fragment bind the promoter-specific cellular transcription factor Sp1 and are protected in DNase protection ("footprinting") experiments. As in SV40, binding occurs in regions containing multiple copies of the sequence GGGCGG. These regions, when fused to the proximal, or "TATA box," element of the herpes simplex virus thymidine kinase promoter, are able to direct Sp1-dependent transcription in vitro. The finding that Sp1 is capable of productive interaction with sequences taken from a cellular promoter supports the idea that Sp1 may play a role in modulating transcription of cellular genes.

摘要

一段440个碱基对的非洲绿猴基因组DNA片段与猿猴病毒40（SV40）的转录调控区域具有同源性，并且据报道在体内可指导转录。我们发现该片段内的两个区域可结合启动子特异性细胞转录因子Sp1，并且在DNA酶保护（“足迹”）实验中受到保护。与SV40一样，结合发生在含有多个GGGCGG序列拷贝的区域。这些区域与单纯疱疹病毒胸苷激酶启动子的近端或“TATA盒”元件融合后，能够在体外指导Sp1依赖性转录。Sp1能够与来自细胞启动子的序列进行有效相互作用这一发现支持了Sp1可能在调节细胞基因转录中发挥作用的观点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/f6e8f26163bc/pnas00355-0063-g.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/50aa36cf0e0e/pnas00355-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/a4d0bca9d933/pnas00355-0061-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/b63cce6076e0/pnas00355-0061-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/caaaffa051b8/pnas00355-0061-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/89841b9030c0/pnas00355-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/dfad40a0be92/pnas00355-0062-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/84d5881419e0/pnas00355-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/d6bc0bef3367/pnas00355-0063-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/5fff030db177/pnas00355-0063-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/219d20dd0787/pnas00355-0063-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/fd41b33d8cab/pnas00355-0063-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/adc9943c8243/pnas00355-0063-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/f6e8f26163bc/pnas00355-0063-g.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/50aa36cf0e0e/pnas00355-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/a4d0bca9d933/pnas00355-0061-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/b63cce6076e0/pnas00355-0061-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/caaaffa051b8/pnas00355-0061-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/89841b9030c0/pnas00355-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/dfad40a0be92/pnas00355-0062-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/84d5881419e0/pnas00355-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/d6bc0bef3367/pnas00355-0063-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/5fff030db177/pnas00355-0063-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/219d20dd0787/pnas00355-0063-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/fd41b33d8cab/pnas00355-0063-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/adc9943c8243/pnas00355-0063-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58b3/390468/f6e8f26163bc/pnas00355-0063-g.jpg

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DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.腺病毒基因在可溶性全细胞提取物中的DNA依赖性转录。
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Organization and expression of eucaryotic split genes coding for proteins.编码蛋白质的真核生物断裂基因的组织与表达。
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Transcription from SV 40-like monkey DNA sequences.源自猴类SV40样DNA序列的转录。
通过Sp1的翻译后修饰对聚（ADP-核糖）聚合酶-1（PARP-1）基因表达的调控：PARP-1的一种核靶蛋白
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Comparative anatomy of the human APRT gene and enzyme: nucleotide sequence divergence and conservation of a nonrandom CpG dinucleotide arrangement.人类APRT基因与酶的比较解剖学：核苷酸序列差异以及非随机CpG二核苷酸排列的保守性
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Compilation of transcription regulating proteins.转录调节蛋白的汇编
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Nucleic Acids Res. 1983 Apr 25;11(8):2447-64. doi: 10.1093/nar/11.8.2447.
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J Mol Appl Genet. 1982;1(5):457-81.