Jones K A, Tjian R
Nature. 1985;317(6033):179-82. doi: 10.1038/317179a0.
During a herpes simplex virus (HSV-1) lytic infection, three classes of viral genes are transcribed in a temporally regulated manner. The HSV-1 'immediate-early' (IE) promoter sequences contain multiple copies of a hexanucleotide sequence, GGGCGG, known as a GC box, and one or more copies of an 11-base pair (bp) conserved A + T-rich element, designated TAATGARAT. The TAATGARAT elements are thought to mediate the trans-activation of IE RNA synthesis by a virion-associated protein(s), and the flanking G + C-rich sequences appear both to potentiate this induction and to direct IE promoter activity in vivo. The similarity of the herpesvirus GC box repeats to those of the simian virus 40 (SV40) early promoter prompted the in vitro analysis of HSV IE transcription reported here. We show that the mammalian gene-specific transcription factor Sp1 binds to eight distinct regions of the HSV short terminal repeat and stimulates transcription 25-fold from the divergent IE-3 (ICP-4) and IE-4/5 promoters.
在单纯疱疹病毒1型（HSV - 1）的裂解感染过程中，三类病毒基因按时间顺序进行转录调控。HSV - 1“立即早期”（IE）启动子序列包含多个六核苷酸序列GGGCGG的拷贝，即所谓的GC盒，以及一个或多个11碱基对（bp）的保守富含A + T的元件拷贝，命名为TAATGARAT。TAATGARAT元件被认为通过一种病毒体相关蛋白介导IE RNA合成的反式激活，并且其富含G + C的侧翼序列似乎既能增强这种诱导作用，又能在体内指导IE启动子活性。疱疹病毒GC盒重复序列与猿猴病毒40（SV40）早期启动子重复序列的相似性促使了本文所报道的HSV IE转录的体外分析。我们发现哺乳动物基因特异性转录因子Sp1结合到HSV短末端重复序列的八个不同区域，并从不同的IE - 3（ICP - 4）和IE - 4/5启动子刺激转录25倍。
