Naghizadeh Mohammed, Wattrang Eva, Kjærup Rikke B, Bakke Maja, Shih Sandra, Dalgaard Tina S
a Department of Poultry Science , Tarbiat Modares University , Tehran , Iran.
b Department of Animal Science , Aarhus University , Tjele , Denmark.
Avian Pathol. 2018 Oct;47(5):479-488. doi: 10.1080/03079457.2018.1490006. Epub 2018 Jul 9.
Opsonins, an important arm of the innate immune system, are various soluble proteins, which play a critical role in destruction of invading pathogens directly or via engulfment of pathogens through the intermediate of phagocytosis. The diversity of opsonin profiles is under genetic influence and may be associated with variation in disease resistance. The aim of this study was to set up an assay to determine serum opsonophagocytic potential (OPp) for chicken sera by flow cytometry and to evaluate the assay using samples from different chicken lines. Two chicken lines selected for high and low concentrations of mannose-binding lectin, a known opsonin, in serum were used to establish the method. Furthermore, the presumed "robust" Hellevad chickens and two other commercial chicken lines (Hisex and Bovans) were tested to evaluate OPp as a parameter reflecting general immune competence. The results showed that Hellevad and Bovans chickens had higher OPp than Hisex chickens. There were no correlations between concentrations of total IgY or mannose-binding lectin and OPp. However, a strong positive correlation was observed between vaccine-induced infectious bronchitis virus titres and OPp. Moreover, inverse relationships were observed between concentrations of total serum IgM as well as natural antibody levels, and OPp. In conclusion, in vitro opsonophagocytosis assessment and determination of OPp may be of relevance when addressing general innate immunocompetence. RESEARCH HIGHLIGHTS A flow cytometry method was developed to assess poultry serum opsonophagocytosis potential. This method is based on serum-opsonin-coated polystyrene beads and HD11 cell phagocytosis. Serum samples from different commercial chicken lines were compared. Opsonophagocytic potential may be included in assay panels for general immune competence of poultry.
调理素是天然免疫系统的重要组成部分，是多种可溶性蛋白质，它们在直接破坏入侵病原体或通过吞噬作用吞噬病原体的过程中发挥关键作用。调理素谱的多样性受遗传影响，可能与抗病性的差异有关。本研究的目的是建立一种通过流式细胞术测定鸡血清调理吞噬潜能（OPp）的方法，并使用来自不同鸡品系的样本对该方法进行评估。选择血清中已知调理素甘露糖结合凝集素浓度高和低的两个鸡品系来建立该方法。此外，对假定“健壮”的赫勒瓦德鸡和另外两个商业鸡品系（海赛克斯和博万斯）进行测试，以评估OPp作为反映一般免疫能力的参数。结果表明，赫勒瓦德鸡和博万斯鸡的OPp高于海赛克斯鸡。总IgY或甘露糖结合凝集素的浓度与OPp之间没有相关性。然而，观察到疫苗诱导的传染性支气管炎病毒滴度与OPp之间存在强正相关。此外，观察到血清总IgM浓度以及天然抗体水平与OPp之间呈负相关。总之，在评估一般天然免疫能力时，体外调理吞噬作用评估和OPp的测定可能具有相关性。研究亮点开发了一种流式细胞术方法来评估家禽血清调理吞噬潜能。该方法基于血清调理素包被的聚苯乙烯珠和HD11细胞吞噬作用。比较了不同商业鸡品系的血清样本。调理吞噬潜能可纳入家禽一般免疫能力的检测指标中。
