Department of Chemistry, The Fifth People's Hospital of Shanghai, Institutes of Biomedical Sciences, and Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China.
Nanoscale. 2018 Jul 5;10(25):12149-12155. doi: 10.1039/c8nr03174f.
Hydrophilic interaction liquid chromatography (HILIC) is a useful tool in glycoproteomic analysis. Glutathione (GSH) is a well-known zwitterionic tripeptide with great hydrophilicity and biocompatibility and is ubiquitous in biological activities. In this study, a hydrophilic metal-organic framework (denoted as mMOF@Au@GSH) was synthesized by grafting glutathione on Au-immobilized magnetic MOFs via the affinity between the thiol group in glutathione and Au. Endowed with the high hydrophilicity of glutathione, the large surface area of the MOF and strong magnetic responsiveness of magnetic nanoparticles, the as-prepared mMOF@Au@GSH exhibited high selectivity (1 : 100) and great sensitivity (0.5 fmol μL-1) towards glycopeptides. Furthermore, it also achieved outstanding performance in enriching glycopeptides from complex biological samples. In all, 273 glycopeptides corresponding to 94 glycoproteins were identified from only 2 μL human serum.
亲水作用色谱（HILIC）是糖蛋白质组学分析中的一种有用工具。谷胱甘肽（GSH）是一种众所周知的两性离子三肽，具有极好的亲水性和生物相容性，在生物活性中无处不在。在这项研究中，通过巯基与金之间的亲和力，将谷胱甘肽接枝到金固定在磁性 MOF 上，合成了一种亲水型金属有机骨架（表示为 mMOF@Au@GSH）。由于谷胱甘肽的高亲水性、MOF 的大表面积和磁性纳米粒子的强磁响应性，所制备的 mMOF@Au@GSH 对糖肽表现出高选择性（1:100）和高灵敏度（0.5 fmol μL-1）。此外，它还在从复杂的生物样品中富集糖肽方面表现出了出色的性能。总共从仅 2 μL 的人血清中鉴定出 273 种糖肽，对应 94 种糖蛋白。
