Harada S, Koyanagi Y, Yamamoto N
Science. 1985 Aug 9;229(4713):563-6. doi: 10.1126/science.2992081.
The human T-cell lines MT-2 and MT-4 carry the human T-cell leukemia virus type I (HTLV-I). When MT-2 and MT-4 were infected with HTLV-III, the probable etiologic agent of the acquired immune deficiency syndrome (AIDS), rapid cytopathogenic effects and cytotoxicity were observed that made it possible to titrate the biologically active virus in a plaque-forming assay. The cytopathogenic effects were preceded by the rapid induction and increase of HTLV-III antigens as revealed by immunofluorescence and immunoprecipitation. Activities of HTLV-III were neutralized by the human antibodies against the virus when immunofluorescence and plaque assays were used. Essentially the same results were obtained with the lymphadenopathy-associated virus (LAV1).
人T细胞系MT - 2和MT - 4携带I型人T细胞白血病病毒（HTLV - I）。当MT - 2和MT - 4被后天免疫缺乏综合征（艾滋病）的可能病原体HTLV - III感染时，观察到快速的细胞病变效应和细胞毒性，这使得在空斑形成试验中对生物活性病毒进行滴定成为可能。免疫荧光和免疫沉淀显示，在细胞病变效应出现之前，HTLV - III抗原会迅速诱导并增加。当使用免疫荧光和空斑试验时，HTLV - III的活性被抗该病毒的人抗体中和。用淋巴结病相关病毒（LAV1）也获得了基本相同的结果。
