Nakashima H, Yoshida T, Harada S, Yamamoto N
Int J Cancer. 1986 Sep 15;38(3):433-6. doi: 10.1002/ijc.2910380320.
Effect of human interferon gamma (rINF gamma) on HTLV-III replication was evaluated quantitatively via a novel infection system using HTLV-I-carrying MT-4 cells. Treatment of HTLV-III-infected MT-4 cells with different concentrations (I-1,000 U/ml) of rINF gamma, which did not affect the growth or viability of uninfected cells, significantly blocked the appearance of immunofluorescent antigens of HTLV-III and the virus-induced cytopathic effect in a dose-dependent manner. A plaque assay was applied to measure the exact amount of viral particles released from HTLV-III-infected MT-4 cultures either untreated or treated with rINF gamma after infection. The number of plaques per dish decreased with increasing drug concentrations. About 50% and 80% of HTLV-III replication were inhibited by the addition of 100 and 1,000 U/ml of rINF gamma, respectively. The effects of INF were observed by day 5 of incubation with the chemical. However, longer treatment of cells with rINF gamma permitted a gradual increase in viral replication. Re-addition of fresh INF into cultures did not change this pattern significantly.
通过一种使用携带HTLV-I的MT-4细胞的新型感染系统，对人干扰素γ（rINFγ）对HTLV-III复制的影响进行了定量评估。用不同浓度（1-1000 U/ml）的rINFγ处理HTLV-III感染的MT-4细胞，该浓度对未感染细胞的生长或活力没有影响，以剂量依赖的方式显著阻断了HTLV-III免疫荧光抗原的出现和病毒诱导的细胞病变效应。应用噬斑测定法来测量感染后未处理或用rINFγ处理的HTLV-III感染的MT-4培养物中释放的病毒颗粒的确切数量。每盘噬斑数随药物浓度增加而减少。分别添加100和1000 U/ml的rINFγ可抑制约50%和80%的HTLV-III复制。在与该化学品孵育的第5天观察到INF的作用。然而，用rINFγ对细胞进行更长时间的处理会使病毒复制逐渐增加。向培养物中重新添加新鲜的INF并没有显著改变这种模式。
