Shi Mingmin, Chen Lei, Wang Yangxin, Yan Shigui
1 Department of Orthopaedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
2 Department of Endocrinology and Metabolism, Sir Run Run Shaw Hospital Affiliated with the School of Medicine, Zhejiang University, Hangzhou, China.
J Int Med Res. 2018 Jul;46(7):2803-2809. doi: 10.1177/0300060518773023. Epub 2018 Jun 19.
Objective This study aimed to investigate the effect of low-intensity pulsed ultrasound on antibiotic release from gentamicin-loaded, self-setting calcium phosphate cement. Methods A gentamicin-loaded calcium phosphate cement cylinder was eluted in stimulated body fluid. Low-intensity pulsed ultrasound (46.5 kHz, 200 mW/cm) was used to produce a sinusoidal wave in the experimental group. Non-gentamicin calcium phosphate cement was used in the control group. Results The transient concentration and cumulatively released percentage of gentamicin in the ultrasound group were higher than those in control group at every time point. The duration of gentamicin concentrations over the level of the minimum inhibitory concentration was significantly prolonged in the ultrasound group compared with the control group. Antibacterial efficacy of gentamicin in the ultrasound group was significantly better than that in the control group with the same concentration of gentamicin. Conclusion Low-intensity pulsed ultrasound enhances antibiotic release, providing sustained antibiotic release at high concentrations. This increases the antibacterial effect of gentamicin.
目的 本研究旨在探讨低强度脉冲超声对载有庆大霉素的自固化磷酸钙骨水泥中抗生素释放的影响。方法 将载有庆大霉素的磷酸钙骨水泥圆柱体在模拟体液中洗脱。实验组使用低强度脉冲超声（46.5 kHz，200 mW/cm²）产生正弦波。对照组使用不含庆大霉素的磷酸钙骨水泥。结果 在每个时间点，超声组中庆大霉素的瞬时浓度和累积释放百分比均高于对照组。与对照组相比，超声组中庆大霉素浓度超过最低抑菌浓度水平的持续时间显著延长。在相同庆大霉素浓度下，超声组中庆大霉素的抗菌效果明显优于对照组。结论 低强度脉冲超声可增强抗生素释放，实现高浓度抗生素的持续释放。这增强了庆大霉素的抗菌效果。
