Biological and Health Sciences Ph.D. Program, Metropolitan Autonomous University (Universidad Autónoma Metropolitana) Mexico City, Mexico.
Health and Care Department, Metropolitan Autonomous University (Universidad Autónoma Metropolitana- Iztapalapa) Mexico City, Mexico.
J Biol Regul Homeost Agents. 2018 May-Jun;32(3):479-487.
Alterations in cellular and extracellular matrix components play an important role during tumorigenesis; proteoglycans are included among these components. Ameloblastomas are odontogenic tumors distinguished as invasive and infiltrative neoplasms and are divided into different histological types, the most common of which are the unicystic ameloblastoma and the conventional ameloblastoma. The aim of this study was to identify the presence of two proteoglycans, perlecan and biglycan, in different types of ameloblastoma. Using immunohistochemistry, we determined the presence of both proteins in 28 unicystic ameloblastomas and 23 conventional ameloblastomas. We identified the cytoplasmic and nuclear presence of perlecan and the cytoplasmic presence of biglycan in both types of ameloblastoma. The mean values of immunoexpression were higher in the conventional type compared to the unicystic type. Neither the presence of biglycan in ameloblastomas nor the nuclear presence of perlecan in any odontogenic tumor has previously been reported. The differential immunoexpression of perlecan and biglycan in these types of ameloblastomas suggests their participation in the developmental process of these tumors.
细胞和细胞外基质成分的改变在肿瘤发生过程中起着重要作用；蛋白聚糖就是其中的一种成分。成釉细胞瘤是一种牙源性肿瘤，被分为侵袭性和浸润性肿瘤，并分为不同的组织学类型，其中最常见的是单囊型成釉细胞瘤和常规型成釉细胞瘤。本研究旨在确定两种蛋白聚糖，即核心蛋白聚糖和 biglycan，在不同类型的成釉细胞瘤中的存在情况。通过免疫组织化学方法，我们确定了 28 例单囊型成釉细胞瘤和 23 例常规型成釉细胞瘤中这两种蛋白的存在。我们在这两种成釉细胞瘤中都发现了核心蛋白聚糖的细胞质和核存在，以及 biglycan 的细胞质存在。常规型的免疫表达平均值高于单囊型。以前从未报道过 biglycan 在成釉细胞瘤中的存在，也没有报道过核心蛋白聚糖在任何牙源性肿瘤中的核存在。这两种成釉细胞瘤中核心蛋白聚糖和 biglycan 的差异免疫表达表明它们参与了这些肿瘤的发育过程。
