将感染恰巴疟原虫的红细胞分离为寄生虫和血影。
Fractionation of Plasmodium chabaudi-infected erythrocytes into parasites and ghosts.
作者信息
Wunderlich F, Schillinger G, Helwig M
出版信息
Z Parasitenkd. 1985;71(4):545-51. doi: 10.1007/BF00928358.
We have developed a new procedure for subfractionation of Plasmodium chabaudi-infected erythrocytes into parasites and ghosts. Trophozoite-infected erythrocytes enriched over a percoll-step (:1.10 g/cm3) are subjected to a glycerol-enhanced osmotic shock. This induces the release of parasites and the emergence of erythrocyte ghosts, which can be separated on a continuous percoll gradient (:1.02----1.10 g/cm3). The parasites are intact in terms of ultrastructure and incorporation of 14C-isoleucine. The erythrocyte ghosts are purified over a two-step percoll gradient (:1.01-1.02 g/cm3). Our method recovers about 40%-50% of the initial ghosts and 70%-95% of the initially freed parasites.
我们已开发出一种将感染查巴迪疟原虫的红细胞亚分级为寄生虫和血影的新方法。通过 Percoll 梯度离心（密度为 1.10 g/cm³）富集的滋养体感染红细胞，进行甘油增强的渗透休克处理。这会促使寄生虫释放并形成红细胞血影，二者可在连续的 Percoll 梯度（密度为 1.02 - 1.10 g/cm³）上分离。从超微结构和 14C - 异亮氨酸掺入情况来看，寄生虫是完整的。红细胞血影通过两步 Percoll 梯度离心（密度为 1.01 - 1.02 g/cm³）进行纯化。我们的方法可回收约 40% - 50%的初始血影以及 70% - 95%最初释放的寄生虫。
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