一种从培养物中分离恶性疟原虫活的成熟寄生虫的简单方法。
A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures.
作者信息
Dluzewski A R, Ling I T, Rangachari K, Bates P A, Wilson R J
出版信息
Trans R Soc Trop Med Hyg. 1984;78(5):622-4. doi: 10.1016/0035-9203(84)90221-9.
Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasites. Centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. Washed parasites derived from the layer or pellet showed good viability in vitro.
从含有5%至10%晚期形态的恶性疟原虫有结或无结克隆培养物中收获成熟无性寄生虫，方法是将多达10⁹个红细胞铺在3毫升 Percoll 上。根据寄生虫的成熟度，将 Percoll 的密度调整至1.081至1.091克/立方厘米之间。以1500克离心10分钟，在界面处产生一条清晰的带，其中含有成熟寄生虫，纯度平均为86%。从该层或沉淀中获得的洗涤过的寄生虫在体外显示出良好的活力。
Zotero 插件