Tarifeño-Saldivia Estefanía, Aguilar Andrea, Contreras David, Mercado Luis, Morales-Lange Byron, Márquez Katherine, Henríquez Adolfo, Riquelme-Vidal Camila, Boltana Sebastian
Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, University of Concepcion, Concepción, Chile.
Interdisciplinary Center for Aquaculture Research (INCAR), Department of Oceanography, Biotechnology Center, University of Concepción, Concepción, Chile.
Front Immunol. 2018 Jun 5;9:1296. doi: 10.3389/fimmu.2018.01296. eCollection 2018.
Iron is a trace element, essential to support life due to its inherent ability to exchange electrons with a variety of molecules. The use of iron as a cofactor in basic metabolic pathways is essential to both pathogenic microorganisms and their hosts. During evolution, the shared requirement of micro- and macro-organisms for this important nutrient has shaped the pathogen-host relationship. Infectious pancreatic necrosis virus (IPNv) affects salmonids constituting a sanitary problem for this industry as it has an important impact on post-smolt survival. While immune modulation induced by IPNv infection has been widely characterized on , viral impact on iron host metabolism has not yet been elucidated. In the present work, we evaluate short-term effect of IPNv on several infected tissues from . We observed that IPNv displayed high tropism to headkidney, which directly correlates with a rise in oxidative stress and antiviral responses. Transcriptional profiling on headkidney showed a massive modulation of gene expression, from which biological pathways involved with iron metabolism were remarkable. Our findings suggest that IPNv infection increase oxidative stress on headkidney as a consequence of iron overload induced by a massive upregulation of genes involved in iron metabolism.
铁是一种微量元素，由于其具有与多种分子交换电子的内在能力，对维持生命至关重要。铁作为基本代谢途径中的辅因子，对致病微生物及其宿主都至关重要。在进化过程中，微生物和宏观生物对这种重要营养素的共同需求塑造了病原体与宿主的关系。传染性胰腺坏死病毒（IPNv）感染鲑科鱼类，对该行业构成卫生问题，因为它对养殖后幼鲑的存活有重要影响。虽然IPNv感染诱导的免疫调节已得到广泛研究，但病毒对宿主铁代谢的影响尚未阐明。在本研究中，我们评估了IPNv对来自[具体来源未提及]的几种受感染组织的短期影响。我们观察到IPNv对头肾表现出高度嗜性，这与氧化应激和抗病毒反应的增加直接相关。对头肾的转录谱分析显示基因表达发生了大规模调节，其中与铁代谢相关的生物学途径显著。我们的研究结果表明，由于参与铁代谢的基因大量上调导致铁过载，IPNv感染会增加头肾的氧化应激。
