源自脂肪组织的间充质干细胞对结肠癌治疗的一种解决方案。
An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue.
作者信息
Iplik Elif Sinem, Ertugrul Baris, Kozanoglu Ilknur, Baran Yusuf, Cakmakoglu Bedia
机构信息
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Istanbul Yeni Yuzyil University, Istanbul, Turkey.
Department of Molecular Medicine, Aziz Sancar Institute for Experimental Medicine, Istanbul University, Istanbul, Turkey.
出版信息
Iran J Basic Med Sci. 2018 May;21(5):465-468. doi: 10.22038/IJBMS.2018.26152.6420.
OBJECTIVES
Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer.
MATERIALS AND METHODS
MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies. MSCs, HT-29, HCT-116, RKO and healthy cell line MRC5 were cultured by different seeding procedure. After cell viability assay, changes in caspase 3 enzyme activity and the level of phosphatidylserine were measured.
RESULTS
For cell viability assay, a 48 hr incubation period was chosen to seed all cells together. There was a 1.36-fold decrease in caspase 3 enzyme activity by co-treatment of RKO and MSCs in addition to 2.02-fold decrease in HT-29 and MSCs co-treatment, and 1.103-fold increase in HCT-116 and MSCs. The results demonstrated that HCT-116 led to the highest rate of apoptotic cell death (7.5%) compared with other cells.
CONCLUSION
We suggest that MSCs might remain a new treatment option for cancer by its differentiation and repair capacity.
目的
结肠癌发病率上升，其机制复杂，直接影响其治疗及常见患病率。间充质干细胞（MSCs）已被视为包括癌症在内的传统疾病的治疗候选者。在本研究中，我们聚焦于脂肪组织来源的间充质干细胞对结肠癌的凋亡作用。
材料与方法
从脂肪组织中获取间充质干细胞，并用合适的抗体通过流式细胞仪进行鉴定。间充质干细胞、HT-29、HCT-116、RKO和健康细胞系MRC5采用不同的接种程序进行培养。在细胞活力测定后，测量半胱天冬酶3酶活性的变化和磷脂酰丝氨酸水平。
结果
对于细胞活力测定，选择48小时的孵育期将所有细胞一起接种。RKO与间充质干细胞共同处理后，半胱天冬酶3酶活性降低了1.36倍，HT-29与间充质干细胞共同处理后降低了2.02倍，HCT-116与间充质干细胞共同处理后增加了1.103倍。结果表明，与其他细胞相比，HCT-116导致的凋亡细胞死亡率最高（7.5%）。
结论
我们认为，间充质干细胞因其分化和修复能力可能仍是癌症的一种新的治疗选择。
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