State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Front Cell Infect Microbiol. 2018 Jun 5;8:189. doi: 10.3389/fcimb.2018.00189. eCollection 2018.
poses a great threat to human health, with no approved vaccine available for the treatment of infection. infections are not limited to the brain, and may also affect other organs especially the liver. Identification of host liver molecules or pathways involved in replication process may lead to the discovery of novel anti- targets. Here, we analyzed the metabolic profile of the liver of mice on 11 and 30 days postinfection (dpi) with type II Pru strain. Global metabolomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) identified 389 significant metabolites from acutely infected mice; and 368 from chronically infected mice, when compared with control mice. Multivariate statistical analysis revealed distinct metabolic signatures from acutely infected, chronically infected and control mice. Infection influenced several metabolic processes, in particular those for lipids and amino acids. Metabolic pathways, such as steroid hormone biosynthesis, primary bile acid biosynthesis, bile secretion, and biosynthesis of unsaturated fatty acids were perturbed during the whole infection process, particularly during the acute stage of infection. The present results provide insight into hepatic metabolic changes that occur in BALB/c mice during acute and chronic infection.
引起了巨大的威胁人类健康，没有批准的疫苗可用于治疗 感染。 感染不仅局限于大脑，还可能影响其他器官，特别是肝脏。鉴定宿主肝脏分子或途径参与 复制过程可能会发现新的抗靶标。在这里，我们分析了感染后第 11 天和第 30 天的 II 型 Pru 株感染小鼠的肝脏代谢谱。使用液相色谱-串联质谱（LC-MS/MS）的全局代谢组学鉴定出 389 种从急性感染小鼠中分离出来的显著代谢物；从慢性感染小鼠中分离出 368 种，与对照组相比。多变量统计分析显示，急性感染、慢性感染和对照组小鼠具有明显不同的代谢特征。感染影响了几种代谢过程，特别是脂质和氨基酸的代谢。在整个感染过程中，特别是在感染的急性期，类固醇激素生物合成、初级胆汁酸生物合成、胆汁分泌和不饱和脂肪酸生物合成等代谢途径发生了紊乱。本研究结果为 BALB/c 小鼠在急性和慢性 感染过程中发生的肝脏代谢变化提供了新的见解。
