Salisu Ibrahim B, Shahid Ahmad A, Ali Qasim, Rao Abdul Q, Husnain Tayyab
Department of Animal Science, Faculty of Agriculture, Federal University Dutse, Dutse, Nigeria.
Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.
Front Nutr. 2018 Jun 5;5:49. doi: 10.3389/fnut.2018.00049. eCollection 2018.
In recent years, the influence of genetically modified (GM) cotton expressing different types of and genes has been attested in term of reduced application of pesticides and insecticides coupled with improved cotton production. Although the cultivation of GM cotton has been authorized by the regulatory authorities of various countries in the world, based on the biosafety studies reported by most of the GM cotton producers, yet the information on its safe use are inadequate. In order to support the issues on food safety, it is therefore mandatory to conduct further safety assessment studies on GM cotton for each independent transgenic event on the basis of case assessment rule. In the present study, the effects of different doses of dietary GM cotton seed expressing and genes were studied on the level of serum biochemical in albino rabbits (). The rabbits were fed a diet containing different levels of GM cotton seeds (i.e., 20, 30, and 40% w/w) respectively mixed with standard diet for 180 days. During the course of the study, various serum enzymes, electrolytes, proteins, glucose and serum total cholesterol were examined at specific time intervals (0, 45, 90, 135, and 180) days. The results showed non-significant ( > 0.05) dose dependent effects in most of the evaluated serum biochemical parameters. Although, the results in some of the serum biochemistry were significantly different ( < 0.05) among the groups, however, they were not biologically significant, since all the differences were within the normal physiological range. These results thus, suggested that the GM cotton seed meal could be considered as safe as other conventional feed ingredients. The experimental evidence for the safe usage of GM cotton was highlighted in this study.
近年来，已证实表达不同类型Bt和CpTI基因的转基因棉花在减少农药和杀虫剂使用以及提高棉花产量方面具有影响力。尽管世界各国的监管机构已批准种植转基因棉花，但根据大多数转基因棉花生产商报告的生物安全研究，其安全使用信息仍不充分。为了支持食品安全问题，因此必须根据个案评估规则，对每个独立的转基因事件进行进一步的转基因棉花安全评估研究。在本研究中，研究了不同剂量的表达Bt和CpTI基因的转基因棉籽对白化兔血清生化水平的影响。分别给兔子喂食含有不同水平转基因棉籽（即20%、30%和40% w/w）并与标准饲料混合的饲料，持续180天。在研究过程中，在特定时间间隔（0、45、90、135和180天）检测各种血清酶、电解质、蛋白质、葡萄糖和血清总胆固醇。结果显示，在大多数评估的血清生化参数中，剂量依赖性影响不显著（P>0.05）。虽然，某些血清生化指标在各组之间存在显著差异（P<0.05），然而，这些差异在生物学上并不显著，因为所有差异都在正常生理范围内。因此，这些结果表明转基因棉籽粕可被视为与其他传统饲料成分一样安全。本研究突出了转基因棉花安全使用的实验证据。
