Division of Animal Nutrition, Central Sheep and Wool Research Institute, Avikanagar Rajasthan, India.
J Anim Physiol Anim Nutr (Berl). 2011 Oct;95(5):545-55. doi: 10.1111/j.1439-0396.2010.01081.x. Epub 2010 Nov 22.
This experiment assessed the effect of feeding genetically modified cottonseed (Bt) containing an insect controlling protein (Cry1Ac) on haematology, blood biochemistry and histopathology of lambs. Haemato-biochemicals were estimated at periodic intervals, and histopathology at termination of experiment. Thirty three weaner lambs were fed a composite feed mixture (CFM) ad libitum individually, in three groups for 123 days. The isonitrogenous CFM had roughage (Perl Millet Stover) and concentrate ratio of 350:650. Diet fed to control lambs contained groundnut oil meal as protein source while other two groups received diet containing either whole cottonseed (N-Bt) or Bt-cottonseed (Bt-CS). Daily feed intake and average daily gain were similar among lambs of three groups. Lambs fed N-Bt diet had higher (p < 0.05) serum protein and globulin compared to control and Bt diets, while albumin content was higher (p = 0.018) in Bt diet fed lambs. Serum urea and creatinin content, alkaline phosphatase (ALP) and serum glutamate pyruvic transaminase (SGPT) activities were not different among lamb groups, while urea and creatinin content and ALP activities increased linearly (p < 0.001) with increased feeding period. Blood haemoglobin (Hb), haematocrit (Hc), white blood cells (WBC), mean corpuscular volume (MCV) and mean corpuscular haemoglobin concentration (MCHC) ranged from 11.1% to 11.2%, 31.8% to 32.8%, 7.0 to 8.3 (× 10(3) /μl), 19.1 to 22.5 fl and 33.2% to 35.5%, respectively, were similar among lamb groups. Erythrocyte sedimentation rate (ESR) (p = 0.008) and red blood cell counts (p = 0.006) were higher in Bt diet fed lambs. Control and N-Bt diet fed lambs had mild fatty infiltration in liver and/or micro-calculi in renal cortex, and such lesions were not seen in Bt diet fed lambs. Growth, haemato-biochemical and histopathology did not change by Bt-CS feeding in growing lamb. However, before recommending the use of Bt-CS in routine feed formulations prolonged feeding experiments of Bt-cotton seed require.
本实验评估了喂养含有昆虫控制蛋白（Cry1Ac）的转基因棉籽（Bt）对羔羊血液学、血液生化和组织病理学的影响。每隔一段时间评估血液生化指标，实验结束时评估组织病理学。33 只断奶羔羊自由采食复合饲料混合物（CFM），单独分为三组，共 123 天。这种等氮 CFM 的粗饲料（珍珠粟秸秆）和精料比例为 350:650。对照组羔羊的饲料以花生油粉作为蛋白质来源，而另外两组则分别以全棉籽（N-Bt）或 Bt 棉籽（Bt-CS）作为饲料。三组羔羊的日采食量和平均日增重相似。与对照组和 Bt 组相比，N-Bt 组羔羊的血清蛋白和球蛋白水平更高（p < 0.05），而 Bt 组羔羊的白蛋白含量更高（p = 0.018）。三组羔羊的血清尿素和肌酐含量、碱性磷酸酶（ALP）和血清谷氨酸丙酮酸转氨酶（SGPT）活性无差异，随着饲养期的延长，尿素和肌酐含量以及 ALP 活性呈线性增加（p < 0.001）。血液血红蛋白（Hb）、红细胞压积（Hc）、白细胞（WBC）、平均红细胞体积（MCV）和平均红细胞血红蛋白浓度（MCHC）分别为 11.1%至 11.2%、31.8%至 32.8%、7.0 至 8.3（×103/μl）、19.1 至 22.5fl 和 33.2%至 35.5%，三组羔羊相似。Bt 组羔羊的红细胞沉降率（ESR）（p = 0.008）和红细胞计数（p = 0.006）较高。对照组和 N-Bt 组羔羊的肝脏有轻度脂肪浸润和/或肾皮质有微结石，而 Bt 组羔羊则没有这些病变。Bt-CS 喂养对生长羔羊的生长、血液生化和组织病理学没有影响。然而，在推荐将 Bt-CS 常规应用于饲料配方之前，需要进行延长的 Bt 棉籽喂养实验。
