碘化丙啶和S1核酸酶：研究DNA重缔合动力学的工具。

Propidium iodide and S1 nuclease: tools for studying DNA reassociation kinetics.

作者信息

Davies W, Nordby O

出版信息

Anal Biochem. 1985 May 1;146(2):423-8. doi: 10.1016/0003-2697(85)90562-7.

Abstract

A method for the determination of the amount of double-stranded DNA in a reassociation mixture is described. Reassociated DNA resistant to S1 nuclease digestion is measured fluorometrically using propidium iodide. A direct comparison is made between this method and an established method in which radiolabeled Escherichia coli DNA resistant to S1 digestion is measured by scintillation counting after separation of nucleotides by Sephadex G-100 chromatography. Reassociation curves determined for calf thymus and E. coli DNA are presented.

摘要

本文描述了一种测定重缔合混合物中双链DNA含量的方法。使用碘化丙啶通过荧光法测量对S1核酸酶消化具有抗性的重缔合DNA。将该方法与一种既定方法进行了直接比较，在既定方法中，对S1消化具有抗性的放射性标记大肠杆菌DNA在通过Sephadex G - 100色谱分离核苷酸后通过闪烁计数进行测量。给出了针对小牛胸腺DNA和大肠杆菌DNA测定的重缔合曲线。