Burr H E, Schimke R T
Nucleic Acids Res. 1982 Jan 22;10(2):719-33. doi: 10.1093/nar/10.2.719.
Reduced-stringency DNA reassociation conditions allow low stability duplexes to be detected in prokaryotic, plant, fish, avian, mammalian, and primate genomes. Highly diverged families of sequences can be detected in avian, mouse, and human unique sequence dNAs. Such a family has been described among twelve species of birds; based on species specific melting profiles and fractionation of sequences belonging to this family, it was concluded that permissive reassociation conditions did not artifactually produce low stability structures (1). We report S1 nuclease and optical melting experiments, and further fractionation of the diverged family to confirm sequence specific DNA reassociation at 50 degrees in 0.5 M phosphate buffer.
降低严格度的DNA重缔合条件可使原核生物、植物、鱼类、鸟类、哺乳动物和灵长类基因组中低稳定性双链体得以检测。在鸟类、小鼠和人类的单拷贝序列DNA中可检测到高度分化的序列家族。在十二种鸟类中已描述了这样一个家族；基于物种特异性解链图谱以及属于该家族序列的分级分离，得出的结论是，宽松的重缔合条件不会人为地产生低稳定性结构(1)。我们报告了S1核酸酶和光学解链实验，以及对该分化家族的进一步分级分离，以证实0.5M磷酸盐缓冲液中50摄氏度时序列特异性DNA重缔合。
