[Correlation of Genetic Polymorphism, Alcoholic Beverage Type and Ethanol Metabolism].

OBJECTIVES

To explore the effects of and gene polymorphism and type of alcoholic beverage on ethanol metabolism, to provide data support for cases involving the interpretation of ethanol metabolism or back calculation of blood ethanol concentration in forensic practice.

METHODS

A total of 81 volunteers were selected. The genotypes of , and were obtained by a multiplex SNaPshot genotyping method. Each subject was administered with 1.0 g/kg of alcohol. About 1 mL venous blood was collected before and after the alcohol consumption at 30 min, 45 min, 1 h, 1.5 h, 2 h, 3 h, 4 h, 5 h, 6 h, 7 h and 8 h, respectively. The concentrations of ethanol and acetaldehyde in blood were determined by headspace gas chromatography. The peak times of blood ethanol concentration （）, the peak mass concentrations of ethanol （）, the area under curve （AUC） of ethanol （AUC）, AUC and ethanol elimination rates （） were calculated. In order to eliminate the influence of , the carriers were grouped based on the genotype of and . The data of each group were evaluated by one-way analysis of variance and pairwise comparison tests were performed by least significant difference method. The gene interactions were evaluated by two-way analysis of variance. Each parameter of three kinds of alcoholic beverage （white wine, red wine and beer） among groups was analysed by variance analysis with randomized block design.

RESULTS

There were no differences in the value of and between the groups with different and genotype. The differences in the values of AUC, and AUC among some groups carrying different and genotype had statistical significance, while no significant difference was observed in these parameters when one individual taking same dose of different alcoholic beverage type.

CONCLUSIONS

The ethanol metabolism is associated with the related gene polymorphism, which is barely affected by alcoholic beverage type.