Research Center for Eco-Environmental Sciences , Chinese Academy of Sciences , 18 Shuangqing Road , Haidian District, Beijing , 100085 , P.R. China.
Benxi institute for Drug Control , No. 31 Shengli Road , Mingshan District, Benxi 117000 , P.R. China.
J Agric Food Chem. 2018 Jul 18;66(28):7376-7383. doi: 10.1021/acs.jafc.8b02102. Epub 2018 Jul 9.
Systematic evaluation of the metabolism, distribution, and effect of thiamethoxam in Mongolian racerunner ( Eremias argus) was carried out after oral exposure. HPLC equipped with Q Exactive focus was used for identification and concentration analysis of thiamethoxam and its metabolites. Percutaneous and urine excretions were the primary ways for the elimination of thiamethoxam and its metabolites, and the limiting factor was urine output. Demethylated thiamethoxam and clothianidin were the main metabolites of thiamethoxam in lizards. CYP3A4, CYP3A7, and CYP2C9 played a crucial role in the metabolism process. Aldehyde oxidase only dominated the nitro-reduction process of demethylated thiamethoxam and clothianidin. Glutathione S-transferase might be related to the clearance process of thiamethoxam and its metabolites. The findings indicated that thiamethoxam might pose potential carcinogenic and hepatic injury risk to lizards. The results enrich and supplement the knowledge of the environmental fate of thiamethoxam in reptiles.
经口暴露后，对噻虫嗪在蒙古沙蜥（Eremias argus）中的代谢、分布和效应进行了系统评价。采用配备 Q Exactive focus 的 HPLC 对噻虫嗪及其代谢物进行了鉴定和浓度分析。噻虫嗪及其代谢物的主要消除途径为经皮和尿排泄，其限制因素为尿排量。去甲基噻虫嗪和噻虫胺是噻虫嗪在蜥蜴中的主要代谢物。CYP3A4、CYP3A7 和 CYP2C9 在代谢过程中起关键作用。醛氧化酶仅主导去甲基噻虫嗪和噻虫胺的硝基还原过程。谷胱甘肽 S-转移酶可能与噻虫嗪及其代谢物的清除过程有关。研究结果表明，噻虫嗪可能对蜥蜴具有潜在的致癌和肝损伤风险。研究结果丰富和补充了噻虫嗪在爬行动物中环境归宿的知识。
