Grand-Perret Virginie, Jacquet Jean-René, Leguerney Ingrid, Benatsou Baya, Grégoire Jean-Marc, Willoquet Georges, Bouakaz Ayache, Lassau Nathalie, Pitre-Champagnat Stephanie
1 Imagerie par Resonance Magnetique Medicale et Multi-Modalites, Université Paris-Saclay, Orsay, France.
2 Imagerie et cerveau, Inserm, Univ. François Rabelais, Tours, France.
Ultrason Imaging. 2018 Sep;40(5):325-338. doi: 10.1177/0161734618783975. Epub 2018 Jun 20.
Tumor microvascularization is a biomarker of response to antiangiogenic treatments and is accurately assessed by ultrasound imaging. Imaging modes used to visualize slow flows include Power Doppler imaging, dynamic contrast-enhanced ultrasonography, and more recently, microvascular Doppler. Flow phantoms are used to evaluate the performance of Doppler imaging techniques, but they do not have a steady flow and sufficiently small channels. We report a novel device for robust and stable microflow measurements and the study of the microvascularization. Based on microfluidics technology, the prototype features wall-less cylindrical channels of diameters ranging from as small as 147 up to 436 µm, cast in a soft silicone polymer and perfused via a microfluidic flow pressure controller. The device was assessed using flow rates from 49 to 146 µL/min, with less than 1% coefficient of variation over three minutes, corresponding to velocities of 6 to 142 mm/s. This enabled us to evaluate and confirm the reliability of the Superb Microvascular Imaging Doppler mode compared with the Power Doppler mode at these flow rates in the presence of vibrations mimicking physiological motion.
肿瘤微血管生成是抗血管生成治疗反应的一个生物标志物，可通过超声成像准确评估。用于可视化缓慢血流的成像模式包括功率多普勒成像、动态对比增强超声检查，以及最近出现的微血管多普勒。血流模拟体用于评估多普勒成像技术的性能，但它们没有稳定的血流和足够小的通道。我们报告了一种用于稳健且稳定的微流测量和微血管生成研究的新型装置。基于微流控技术，该原型具有直径范围小至147 µm至436 µm的无壁圆柱形通道，由软硅聚合物浇铸而成，并通过微流控流动压力控制器进行灌注。该装置使用49至146 µL/分钟的流速进行评估，三分钟内变异系数小于1%，对应速度为6至142 mm/s。这使我们能够在存在模拟生理运动的振动情况下，评估并确认在这些流速下，与功率多普勒模式相比，超微血管成像多普勒模式的可靠性。
