[Isolation and purification of restriction endonuclease PmiI from Proteus mirabilis 1667].
作者信息
Bakh N L, Tsvetkova N V, Semina I E, Tarasov A P, Mileĭkovskaia M M
出版信息
Antibiot Med Biotekhnol. 1985 May;30(5):342-4.
PMID:2992364
Abstract
A new restriction endonuclease Pmi I was detected in Proteus mirabilis 1667. The enzyme hydrolyzes DNA of the phage lambda into 10 electrophoretically separating fragments with molecular weights of 1.3-7.9 mD. With the use of two-stage chromatography on blue sepharose and phosphocellulose it is possible to obtain restriction endonuclease Pmi I free of the admixtures of ballast proteins, nonspecific nucleases and phosphatases.
摘要
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