Racker E, Riegler C
Arch Biochem Biophys. 1985 Aug 1;240(2):836-42. doi: 10.1016/0003-9861(85)90093-1.
Glycolysis of 3T3 and Ehrlich ascites tumor cells was greatly enhanced by Nonidet P-40 or Triton X-100 at about 100 micrograms/mg cell protein. This enhanced glycolysis was partly sensitive to rutamycin and partly sensitive to ouabain, suggesting that the detergent released the control of the ATPase of the mitochondria and of the plasma membrane Na+K+-ATPase. Nonidet P-40 had no effect on glycolysis in cell-free extracts from Ehrlich ascites tumor cells to which soluble mitochondrial ATPase was added. Measuring ouabain-sensitive 22Na efflux and using ouabain-sensitive lactate production as a measure of ATP hydrolysis by the Na+K+ pump, it was shown that Nonidet P-40 greatly decreased the efficiency of the Na+K+ pump. Quercetin increased the efficiency of pumping in EAT cells both in the absence and presence of the detergent.
在浓度约为100微克/毫克细胞蛋白时，去氧胆酸钠或曲拉通X - 100可极大增强3T3细胞和艾氏腹水瘤细胞的糖酵解。这种增强的糖酵解部分对鲁塔霉素敏感，部分对哇巴因敏感，这表明去污剂解除了线粒体ATP酶和质膜钠钾ATP酶的调控。去氧胆酸钠对添加了可溶性线粒体ATP酶的艾氏腹水瘤细胞无细胞提取物中的糖酵解没有影响。通过测量哇巴因敏感的22Na外流，并将哇巴因敏感的乳酸生成作为钠钾泵ATP水解的指标，结果表明去氧胆酸钠极大降低了钠钾泵的效率。无论有无去污剂存在，槲皮素均可提高艾氏腹水瘤细胞的泵浦效率。
