Balaban R S, Bader J P
Biochim Biophys Acta. 1984 Aug 17;804(4):419-26. doi: 10.1016/0167-4889(84)90069-7.
In several tissues a coupling between glycolysis and (Na+ + K+)-ATPase has been observed. We report here studies on the coupling of glycolysis and (Na+ + K+)-ATPase in Rous-transformed hamster cells and Ehrlich ascites tumor cells. The rate of (Na+ + K+)-ATPase was estimated by the initial rate of ouabain-sensitive K+ influx after K+ reintroduction to K+-depleted cells. Experiments were performed with cells producing ATP via oxidative phosphorylation alone (i.e., lactate sole substrate), glycolysis alone (i.e., glucose as substrate in the absence of oxygen or with antimycin A), or glycolysis and oxidative phosphorylation (i.e., glucose as substrate in the presence of oxygen). The cells produced ATP at approximately the same rate under all of these conditions, but the initial rate of K+-influx was approx. 2-fold higher when AtP was produced from glycolysis. Changes in cell Na+ due to other transport processes related to glycolysis, such as Na+-H+ exchange, Na+-glucose cotransport, and K+-H+ exchange were ruled out as mediators of this effect on (Na+ + K+)-ATPase. These data suggest that glycolysis is more effective than oxidative phosphorylation in providing ATP to (Na+ + K+)-ATPase to these cultured cells.
在几种组织中，已观察到糖酵解与（Na⁺ + K⁺）-ATP酶之间存在偶联。我们在此报告关于劳氏肉瘤病毒转化的仓鼠细胞和艾氏腹水瘤细胞中糖酵解与（Na⁺ + K⁺）-ATP酶偶联的研究。通过将钾重新引入低钾细胞后哇巴因敏感的钾流入的初始速率来估计（Na⁺ + K⁺）-ATP酶的速率。实验是在仅通过氧化磷酸化产生ATP的细胞（即仅以乳酸为底物）、仅通过糖酵解产生ATP的细胞（即在无氧或存在抗霉素A的情况下以葡萄糖为底物）或糖酵解与氧化磷酸化同时进行的细胞（即在有氧情况下以葡萄糖为底物）中进行的。在所有这些条件下，细胞产生ATP的速率大致相同，但当通过糖酵解产生ATP时，钾流入的初始速率约高2倍。由于与糖酵解相关的其他转运过程（如Na⁺-H⁺交换、Na⁺-葡萄糖共转运和K⁺-H⁺交换）导致的细胞内钠离子变化被排除为对（Na⁺ + K⁺）-ATP酶产生这种影响的介质。这些数据表明，在为这些培养细胞的（Na⁺ + K⁺）-ATP酶提供ATP方面，糖酵解比氧化磷酸化更有效。
