Betsholtz C, Bywater M, Westermark B, Bürk R R, Heldin C H
Biochem Biophys Res Commun. 1985 Jul 31;130(2):753-60. doi: 10.1016/0006-291x(85)90480-2.
SV40-transformed BHK cells were shown to express two transcripts, of 3.5 kb and 2.0 kb, that hybridised to a human c-sis probe. Antibodies directed against human PDGF specifically recognized a 31 kDa protein in SV40/BHK cell conditioned medium, which upon reduction was split into 16 kDa species. Unfractionated conditioned medium and one of two growth factors isolated from SV40/BHK cells competed with 125I-PDGF for binding to its receptor. The present communication thus provides compelling evidence that an SV40/BHK cell-derived growth factor is a hamster equivalent to human PDGF.
研究表明，经SV40转化的BHK细胞表达两种转录本，大小分别为3.5 kb和2.0 kb，它们能与人c-sis探针杂交。针对人血小板衍生生长因子（PDGF）的抗体能特异性识别SV40/BHK细胞条件培养基中的一种31 kDa蛋白，该蛋白经还原后可裂解为16 kDa的片段。未分级的条件培养基以及从SV40/BHK细胞中分离出的两种生长因子之一，能与125I-PDGF竞争结合其受体。因此，本通讯提供了有力证据，表明一种源自SV40/BHK细胞的生长因子是仓鼠版的人PDGF。
